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. 2019 Oct 19;10(10):362.
doi: 10.3390/insects10100362.

Ιnteractions Between Beauveria bassiana and Isaria fumosorosea and Their Hosts Sitophilus granarius (L.) and Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

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Ιnteractions Between Beauveria bassiana and Isaria fumosorosea and Their Hosts Sitophilus granarius (L.) and Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

Spiridon Mantzoukas et al. Insects. .
Free PMC article

Abstract

The interactions between the entomopathogenic fungus Beauveria bassiana Balsamo (Vuillemin) (Hypocreales: Cordycipitaceae) and the entomopathogenic fungus Isaria fumosorosea (Wize) Brown and Smith (Hypocreales: Clavicipitaceae) were examined on young adults of Sitophilus granarius (L.) (Coleoptera: Curculionidae) and S. oryzae (L.) (Coleoptera: Curculionidae). Conidial suspensions of these entomopathogenic fungi were applied both separately and in combination, at three dosages, 104, 106, and 108 conidia/mL. Mortality of experimental adults was recorded daily for 15 days. An overall positive interaction between the pathogenic microorganisms was observed. Mean weevil mortality caused by the separate acting fungi, B. bassiana, ranged from 26.7% to 53.3% and from 36.6% to 63.3% for S. granarius and S. oryzae, respectively. The respective values for I. fumosorosea were 20.0%-53.3% and 46.7%-66.7%. The combined treatments showed a distinct interaction between the pathogens; for S. granarius, the interaction between the pathogens was additive in all combinations, whereas, for S. oryzae, the interaction was additive in seven and competitive in two of the combinations. Applying both entomopathogenic microorganisms may offer a method for weevil control that could be more effective than using each pathogen alone.

Keywords: Beauveria bassiana; Isaria fumosorosea; Sitophilus granarius; Sitophilus oryzae; interaction.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Percent infection attributed to each insect pathogen after the combined inoculation of Hypocreales fungi on the adults of S. granarius (a) and S. oryzae (b). If—I. fumosorosea; Bb—B. bassiana. Combined Treatment: A: 108 Bb × 104 If, B: 106 Bb × 106 If, C: 104 Bb × 108 If, D: 108 Bb × 106 If, E: 106 Bb × 108 If, F: 104 Bb × 104 If, G: 108 Bb × 108 If, H: 104 Bb × 106 If, I: 106 Bb × 104 If.
Figure 1
Figure 1
Percent infection attributed to each insect pathogen after the combined inoculation of Hypocreales fungi on the adults of S. granarius (a) and S. oryzae (b). If—I. fumosorosea; Bb—B. bassiana. Combined Treatment: A: 108 Bb × 104 If, B: 106 Bb × 106 If, C: 104 Bb × 108 If, D: 108 Bb × 106 If, E: 106 Bb × 108 If, F: 104 Bb × 104 If, G: 108 Bb × 108 If, H: 104 Bb × 106 If, I: 106 Bb × 104 If.
Figure 2
Figure 2
Overall survival of the infected adults of S. granarius and S. oryzae was monitored for 15 days, after being treated with B. bassiana (Bb) and I. fumosorosea (If) (chi-square: 7.630; df: 1, p = 0.006).

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