A super-resolution platform for correlative live single-molecule imaging and STED microscopy

doi:10.1038/s41592-019-0611-8

. 2019 Dec;16(12):1263-1268.

doi: 10.1038/s41592-019-0611-8. Epub 2019 Oct 21.

A super-resolution platform for correlative live single-molecule imaging and STED microscopy

V V G Krishna Inavalli^{1

2}, Martin O Lenz^{1

2}, Corey Butler^{1

2

3}, Julie Angibaud^{1

2}, Benjamin Compans^{1

2}, Florian Levet^{1

2

4}, Jan Tønnesen^{1

2}, Olivier Rossier^{1

2}, Gregory Giannone^{1

2}, Olivier Thoumine^{1

2}, Eric Hosy^{1

2}, Daniel Choquet^{1

2

4}, Jean-Baptiste Sibarita^#^{5

6}, U Valentin Nägerl^#^{7

8}

Affiliations

¹ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.
² Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France.
³ Imagine Optic, Orsay, France.
⁴ Bordeaux Imaging Center, CNRS UMS 3420, University of Bordeaux, INSERM US04, Bordeaux, France.
⁵ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. jean-baptiste.sibarita@u-bordeaux.fr.
⁶ Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France. jean-baptiste.sibarita@u-bordeaux.fr.
⁷ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. valentin.nagerl@u-bordeaux.fr.
⁸ Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France. valentin.nagerl@u-bordeaux.fr.

^# Contributed equally.

PMID: 31636458
DOI: 10.1038/s41592-019-0611-8

A super-resolution platform for correlative live single-molecule imaging and STED microscopy

V V G Krishna Inavalli et al. Nat Methods. 2019 Dec.

. 2019 Dec;16(12):1263-1268.

doi: 10.1038/s41592-019-0611-8. Epub 2019 Oct 21.

Authors

Affiliations

¹ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.
² Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France.
³ Imagine Optic, Orsay, France.
⁴ Bordeaux Imaging Center, CNRS UMS 3420, University of Bordeaux, INSERM US04, Bordeaux, France.
⁵ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. jean-baptiste.sibarita@u-bordeaux.fr.
⁶ Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France. jean-baptiste.sibarita@u-bordeaux.fr.
⁷ Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France. valentin.nagerl@u-bordeaux.fr.
⁸ Interdisciplinary Institute for Neuroscience, CNRS UMR 5297, Bordeaux, France. valentin.nagerl@u-bordeaux.fr.

^# Contributed equally.

PMID: 31636458
DOI: 10.1038/s41592-019-0611-8

Abstract

Super-resolution microscopy offers tremendous opportunities to unravel the complex and dynamic architecture of living cells. However, current super-resolution microscopes are well suited for revealing protein distributions or cell morphology, but not both. We present a super-resolution platform that permits correlative single-molecule imaging and stimulated emission depletion microscopy in live cells. It gives nanoscale access to the positions and movements of synaptic proteins within the morphological context of growth cones and dendritic spines.

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[1] Furstenberg, A. & Heilemann, M. Phys. Chem. Chem. Phys. 15, 14919–14930 (2013). - DOI

[2] Furstenberg, A. & Heilemann, M. Phys. Chem. Chem. Phys. 15, 14919–14930 (2013). - DOI

[3] Cognet, L., Leduc, C. & Lounis, B. Curr. Opin. Chem. Biol. 20, 78–85 (2014). - DOI

[4] Cognet, L., Leduc, C. & Lounis, B. Curr. Opin. Chem. Biol. 20, 78–85 (2014). - DOI

[5] Nägerl, U. V., Willig, K. I., Hein, B., Hell, S. W. & Bonhoeffer, T. Proc. Natl Acad. Sci. USA 105, 18982–18987 (2008). - DOI

[6] Nägerl, U. V., Willig, K. I., Hein, B., Hell, S. W. & Bonhoeffer, T. Proc. Natl Acad. Sci. USA 105, 18982–18987 (2008). - DOI

[7] Testa, I. et al. Neuron 75, 992–1000 (2012). - DOI

[8] Testa, I. et al. Neuron 75, 992–1000 (2012). - DOI

[9] Dudok, B. et al. Nat. Neurosci. 18, 75–86 (2015). - DOI

[10] Dudok, B. et al. Nat. Neurosci. 18, 75–86 (2015). - DOI

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A super-resolution platform for correlative live single-molecule imaging and STED microscopy

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A super-resolution platform for correlative live single-molecule imaging and STED microscopy

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Abstract

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