Application of a Fluorescent Probe for the Online Measurement of PM-Bound Reactive Oxygen Species in Chamber and Ambient Studies

Reece Brown; Svetlana Stevanovic; Zachary Brown; Mingfu Cai; Shengzhen Zhou; Wei Song; Xinming Wang; Branka Miljevic; Jun Zhao; Steven Bottle; Zoran Ristovski

doi:10.3390/s19204564

Application of a Fluorescent Probe for the Online Measurement of PM-Bound Reactive Oxygen Species in Chamber and Ambient Studies

Sensors (Basel). 2019 Oct 21;19(20):4564. doi: 10.3390/s19204564.

Authors

Reece Brown¹, Svetlana Stevanovic^{2

3}, Zachary Brown⁴, Mingfu Cai^{5

6}, Shengzhen Zhou⁷, Wei Song⁸, Xinming Wang⁹, Branka Miljevic^{10

11}, Jun Zhao¹², Steven Bottle¹³, Zoran Ristovski^{14

15}

Affiliations

¹ ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia. reece.a.brown@outlook.com.
² ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia. svetlana.stevanovic@deakin.edu.au.
³ School of Engineering, Deakin University, Geelong 3216 VIC, Australia. svetlana.stevanovic@deakin.edu.au.
⁴ ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia. ze.brown@connect.qut.edu.au.
⁵ School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China. mingfu0721@hotmail.com.
⁶ Institute of Tropical and Marine Meteorology/Guangdong Provincial Key Laboratory of Regional Numerical Weather Prediction, CMA, Guangzhou 510640, China. mingfu0721@hotmail.com.
⁷ School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China. zhoushzh3@mail.sysu.edu.cn.
⁸ State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China. songwei@gig.ac.cn.
⁹ State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China. wangxm@gig.ac.cn.
¹⁰ ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia. b.miljevic@qut.edu.au.
¹¹ School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, 4000 QLD, Australia. b.miljevic@qut.edu.au.
¹² School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China. zhaojun23@mail.sysu.edu.cn.
¹³ School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, 4000 QLD, Australia. s.bottle@qut.edu.au.
¹⁴ ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia. z.ristovski@qut.edu.au.
¹⁵ School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, 4000 QLD, Australia. z.ristovski@qut.edu.au.

Abstract

This manuscript details the application of a profluorescent nitroxide (PFN) for the online quantification of radical concentrations on particulate matter (PM) using an improved Particle Into Nitroxide Quencher (PINQ). A miniature flow-through fluorimeter developed specifically for use with the 9,10-bis(phenylethynyl)anthracene-nitroxide (BPEAnit) probe was integrated into the PINQ, along with automated gas phase corrections through periodic high efficiency particle arrestor (HEPA) filtering. The resulting instrument is capable of unattended sampling and was operated with a minimum time resolution of 2.5 min. Details of the fluorimeter design and examples of data processing are provided, and results from a chamber study of side-stream cigarette smoke and ambient monitoring campaign in Guangzhou, China are presented. Primary cigarette smoke was shown to have both short-lived (t_1/2 = 27 min) and long-lived (t_1/2 = indefinite) PM-bound reactive oxygen species (ROS) components which had previously only been observed in secondary organic aerosol (SOA).

Keywords: BPEAnit; aerosol; oxidative stress; particulate matter; profluorescent nitroxide; reactive oxygen species.

Abstract

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