Protein A superantigen: structure, engineering and molecular basis of antibody recognition

Ohan Mazigi; Peter Schofield; David B Langley; Daniel Christ

doi:10.1093/protein/gzz026

Protein A superantigen: structure, engineering and molecular basis of antibody recognition

Protein Eng Des Sel. 2019 Dec 31;32(8):359-366. doi: 10.1093/protein/gzz026.

Authors

Ohan Mazigi^{1

2}, Peter Schofield^{1

2}, David B Langley¹, Daniel Christ^{1

2}

Affiliations

¹ Department of Immunology, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010, Australia.
² Faculty of Medicine, St Vincent's Clinical School, UNSW Sydney, Darlinghurst, Sydney, NSW 2010, Australia.

PMID: 31641749
DOI: 10.1093/protein/gzz026

Abstract

Staphylococcus aureus interacts with the human immune system through the production of secreted factors. Key among these is protein A, a B-cell superantigen capable of interacting with both antibody Fc and VH regions. Here, we review structural and molecular features of this important example of naturally occurring bacterial superantigens, as well as engineered variants and their application in biotechnology.

Keywords: SpA; antibody; antibody Fc; antibody variable domain VH; protein A; protein engineering; superantigen.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amino Acid Sequence
Humans
Immunoglobulin Fc Fragments / chemistry
Immunoglobulin Fc Fragments / genetics
Immunoglobulin Fc Fragments / immunology*
Protein Binding
Protein Engineering / methods
Protein Folding
Sequence Homology, Amino Acid
Staphylococcal Protein A / chemistry
Staphylococcal Protein A / genetics
Staphylococcal Protein A / immunology*
Staphylococcus aureus / genetics
Staphylococcus aureus / immunology*
Staphylococcus aureus / metabolism
Superantigens / chemistry
Superantigens / genetics
Superantigens / immunology*

Substances

Immunoglobulin Fc Fragments
Staphylococcal Protein A
Superantigens