Purification, identification and properties of a new blue pigment produced from Streptomyces sp. A1013Y

Yunping Zhu; Xiaoya Shang; Li Yang; Shenglan Zheng; Kaifeng Liu; Xiuting Li

doi:10.1016/j.foodchem.2019.125600

Purification, identification and properties of a new blue pigment produced from Streptomyces sp. A1013Y

Food Chem. 2020 Mar 5:308:125600. doi: 10.1016/j.foodchem.2019.125600. Epub 2019 Oct 15.

Authors

Yunping Zhu¹, Xiaoya Shang², Li Yang¹, Shenglan Zheng³, Kaifeng Liu³, Xiuting Li⁴

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China.
² Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, China.
³ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), Beijing 100048, China.
⁴ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China; School of Food and Chemical Engineering, Beijing Technology and Business University, No. 33, Fucheng Road, Beijing 100048, China. Electronic address: lixt@th.btbu.edu.cn.

PMID: 31648091
DOI: 10.1016/j.foodchem.2019.125600

Abstract

A novel blue pigment was first isolated from Streptomyces sp. A1013Y. The purified component was identified as 4,8,13-trihydroxy-6,11-dione-trihydrogranaticins A (TDTA). Its physical properties were found to be: Molecular weight 462 Da; Color value, E^0.1%_1cm580 nm = 80; Solubility, it dissolved in organic solvents. In addition, the color of TDTA changed with pH but was found to be relatively stable between 20 and 100 °C, from pH 3 to pH 11, and under UV-light or darkness. TDTA's functional properties was as follows: TDTA showed excellent free radical scavenging properties, IC₅₀ 41.04 µg/mL and 13.75 µg/mL using 2, 20-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-n-(3, 2-ethyl-benzothiazole-6-sulfonic acid) ammonium salt (ABTS) respectively. TDTA might be a promising source of natural pigment and bioactive compound used as additive in food industry.

Keywords: Antibacterial activity; Blue pigment; Radical scavenging activities; Stability; Streptomyces sp. A1013Y.

MeSH terms

Benzothiazoles / chemistry
Biphenyl Compounds / chemistry
Color
Picrates / chemistry
Pigmentation
Solubility
Solvents
Streptomyces / chemistry*

Substances

Benzothiazoles
Biphenyl Compounds
Picrates
Solvents
benzothiazole-2-sulfonic acid
1,1-diphenyl-2-picrylhydrazyl