Objective: To study the preservation effect of true bone ceramics (TBC) prepared by high-temperature calcination of bovine bone on alveolar ridge of canine extraction socket.
Methods: Six healthy Beagle dogs (aged 1.5-2 years) were selected to extract the second and fourth premolars of both mandibles and the second premolars of the maxilla. The left extraction socket was implanted with TBC as the experimental group, and the right side was implanted with the calcined bovine bone (CBB) as the control group, to observe the alveolar ridge preservation effect. Three dogs were euthanized after general observation at 1 and 6 months after operation respectively. After separating the maxilla and mandible, cone beam CT (CBCT) was performed to measure the average gray value of the graft site and the adjacent reference area (the area between the roots of the adjacent third premolar) and calculate the gray scale ratio between the bone graft site and the reference area. Histological observation was made on the bone graft site to evaluate the new bone formation.
Results: General observation showed that the wounds of both groups were basically healed at 2 weeks after operation, and the bone graft materials were not exposed. The wounds healed well at 1 and 6 months after operation without swelling. The results of CBCT showed that the residual material was found in both groups at 1 month after operation, and no significant residual material was found in both groups at 6 months after operation, and the alveolar ridge height of the bone graft area was not significantly reduced. There was no significant difference in the bone mineral density between the experimental group and the control group. The gray scale ratios of the experimental group at 1 month and 6 months after operation were 0.97±0.14 and 0.93±0.06, respectively, and were 0.99±0.16 and 0.94±0.05 in control group, showing no significant difference between the two groups ( t=-1.030, P=0.333; t=-0.770, P=0.466). HE staining observation showed that a large number of bone graft materials did not degrade and new bone formed around the grafts in both groups at 1 month after operation; the bone graft materials were absorbed and a large number of new bones were formed in both groups at 6 months after operation.
Conclusion: TBC can maintain bone mineral density and have good osteoconductivity in the alveolar ridge site preservation experiment of dogs, and can be used for alveolar ridge site preservation.
目的: 研究将牛骨经过高温煅烧制备的煅烧异种骨材料（以下简称煅烧异种骨）用于犬拔牙窝牙槽嵴位点保存的效果。.
方法: 取健康 1.5～2 岁龄比格犬 6 只，拔除双侧下颌第 2、4 前磨牙以及上颌第 2 前磨牙，左侧拔牙窝植入煅烧异种骨作为实验组，右侧植入天然煅烧骨修复材料（商品名：骼瑞）作为对照组。术后 1、6 个月行伤口及植骨材料大体观察后，分别过量麻醉处死 3 只实验动物，分离上下颌骨后行锥形束 CT 检查，测量植骨部位与相邻参照区（邻近的第 3 前磨牙两个牙根中间的骨区）的平均灰度值，计算植骨处与参照区的灰度比；常规行 HE 染色观察两组植入材料吸收情况和新骨形成情况，评价材料成骨效果。.
结果: 大体观察示，术后 2 周实验组和对照组创口均基本愈合，植骨材料无裸露；术后 1、6 个月创口均愈合良好，无肿胀。锥形束 CT 检查示，术后 1 个月，两组植骨区均可见植骨材料残留；术后 6 个月，两组植骨区未见明显植骨材料残留，植骨区牙槽嵴高度均未见明显降低，实验组和对照组牙槽窝内骨量无明显差异。术后 1、6 个月实验组植骨处与对应参照区的灰度比分别为 0.97±0.14、0.93±0.06，对照组分别为 0.99±0.16、0.94±0.05，两组比较差异均无统计学意义（ t=−1.030， P=0.333； t=−0.770， P=0.466）。HE 染色示，术后 1 个月，两组植骨部位均可见大量植骨材料未降解，植骨材料周围可见新生骨组织；术后 6 个月，两组植骨材料基本完全降解，均可见大量成骨。.
结论: 煅烧异种骨材料能够保持牙槽骨密度，并有良好的成骨效果，能够用于牙槽嵴位点保存。.
Keywords: True bone ceramics; alveolar ridge preservation; canine extraction socket filling; gray scale ratio.