Fusaricidin Produced by Paenibacillus polymyxa WLY78 Induces Systemic Resistance against Fusarium Wilt of Cucumber

Int J Mol Sci. 2019 Oct 22;20(20):5240. doi: 10.3390/ijms20205240.

Abstract

Cucumber is an important vegetable crop in China. Fusarium wilt is a soil-borne disease that can significantly reduce cucumber yields. Paenibacillus polymyxa WLY78 can strongly inhibit Fusarium oxysporum f. sp. Cucumerium, which causes Fusarium wilt disease. In this study, we screened the genome of WLY78 and found eight potential antibiotic biosynthesis gene clusters. Mutation analysis showed that among the eight clusters, the fusaricidin synthesis (fus) gene cluster is involved in inhibiting the Fusarium genus, Verticillium albo-atrum, Monilia persoon, Alternaria mali, Botrytis cinereal, and Aspergillus niger. Further mutation analysis revealed that with the exception of fusTE, the seven genes fusG, fusF, fusE, fusD, fusC, fusB, and fusA within the fus cluster were all involved in inhibiting fungi. This is the first time that demonstrated that fusTE was not essential. We first report the inhibitory mode of fusaricidin to inhibit spore germination and disrupt hyphal membranes. A biocontrol assay demonstrated that fusaricidin played a major role in controlling Fusarium wilt disease. Additionally, qRT-PCR demonstrated that fusaricidin could induce systemic resistance via salicylic acid (SA) signal against Fusarium wilt of cucumber. WLY78 is the first reported strain to both produce fusaricidin and fix nitrogen. Therefore, our results demonstrate that WLY78 will have great potential as a biocontrol agent in agriculture.

Keywords: Paenibacillus polymyxa; biocontrol; gene disruption; induced systemic resistance.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Bacterial Proteins / metabolism
  • Bacterial Proteins / pharmacology*
  • Cucumis sativus / immunology
  • Cucumis sativus / microbiology*
  • Depsipeptides / metabolism
  • Depsipeptides / pharmacology*
  • Disease Resistance
  • Fusarium / drug effects*
  • Fusarium / pathogenicity
  • Paenibacillus polymyxa / genetics
  • Paenibacillus polymyxa / metabolism*

Substances

  • Antifungal Agents
  • Bacterial Proteins
  • Depsipeptides