A safe ride in extracellular vesicles - small RNA trafficking between plant hosts and pathogens

Abstract

Communication between plants and pathogens requires the transport of regulatory molecules across cellular boundaries, which is essential for host defense and pathogen virulence. Previous research has largely focused on protein transport, but, which other molecules function in communication, and how they are transported remains under explored. Recent studies discovered that small RNAs (sRNAs) are transported between plants and pathogens, which can silence target genes in the interacting organisms and regulate host immunity and pathogen infection, a mechanism called 'cross-kingdom RNA interference (RNAi)'. Further studies indicate that plant extracellular vesicles (EVs) are essential for sRNA trafficking and host-pathogen communication. This review will focus on the latest advances in our understanding of plant EVs and their roles in transporting regulatory molecules, especially sRNAs, between hosts and pathogens.