A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq

Funct Integr Genomics. 2020 May;20(3):343-353. doi: 10.1007/s10142-019-00719-7. Epub 2019 Oct 28.


Evidence from biochemical liver function index and histopathology analysis suggested that selenium could effectively repair the liver injury caused by beta-cypermethrin (β-CYP). However, the molecular mechanism of selenium against liver injury induced by β-CYP remains unclear. In the present study, dynamic changes in gene expression profiles before and after the treatment of Na2SeO3 in liver injury mice were analyzed by using RNA sequencing. As a result, several essential genes and pathways were identified to be significantly associated with this process. In particular, ten genes including Cyp2j11, Cyp2b10, Cyp3a13, Dhrs9, Socs2, Stat4, Gm13305, Cyp3a44, Retsat, and Cyp26b1 were significantly enriched in the functional categories related to retinol metabolism, linoleic acid metabolism, and Jak-STAT signaling pathway. Among them, the expression patterns of nine genes were validated by qRT-PCR, except for Cyp3a44. Furthermore, we have constructed the associated regulatory network based on the identified targets revealed by high throughput screening. Our study may provide insight into the molecular mechanism underlying the protective effect of selenium against liver injury induced by β-CYP in mammals.

Keywords: Genes; Liver injury; Pathways; RNA sequencing; Selenium.

MeSH terms

  • Animals
  • Chemical and Drug Induced Liver Injury / drug therapy
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / genetics*
  • Chemical and Drug Induced Liver Injury / metabolism
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Insecticides / toxicity
  • Janus Kinases / genetics
  • Janus Kinases / metabolism
  • Liver / drug effects
  • Liver / metabolism*
  • Mice
  • Pyrethrins / toxicity
  • STAT Transcription Factors / genetics
  • STAT Transcription Factors / metabolism
  • Selenium / pharmacology*
  • Selenium / therapeutic use
  • Transcriptome*


  • Insecticides
  • Pyrethrins
  • STAT Transcription Factors
  • cypermethrin
  • Cytochrome P-450 Enzyme System
  • Janus Kinases
  • Selenium