Single-Molecule Analysis and Engineering of DNA Motors

Chem Rev. 2020 Jan 8;120(1):36-78. doi: 10.1021/acs.chemrev.9b00361. Epub 2019 Oct 29.


Molecular motors are diverse enzymes that transduce chemical energy into mechanical work and, in doing so, perform critical cellular functions such as DNA replication and transcription, DNA supercoiling, intracellular transport, and ATP synthesis. Single-molecule techniques have been extensively used to identify structural intermediates in the reaction cycles of molecular motors and to understand how substeps in energy consumption drive transitions between the intermediates. Here, we review a broad spectrum of single-molecule tools and techniques such as optical and magnetic tweezers, atomic force microscopy (AFM), single-molecule fluorescence resonance energy transfer (smFRET), nanopore tweezers, and hybrid techniques that increase the number of observables. These methods enable the manipulation of individual biomolecules via the application of forces and torques and the observation of dynamic conformational changes in single motor complexes. We also review how these techniques have been applied to study various motors such as helicases, DNA and RNA polymerases, topoisomerases, nucleosome remodelers, and motors involved in the condensation, segregation, and digestion of DNA. In-depth analysis of mechanochemical coupling in molecular motors has made the development of artificially engineered motors possible. We review techniques such as mutagenesis, chemical modifications, and optogenetics that have been used to re-engineer existing molecular motors to have, for instance, altered speed, processivity, or functionality. We also discuss how single-molecule analysis of engineered motors allows us to challenge our fundamental understanding of how molecular motors transduce energy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Bioengineering / methods
  • DNA / chemistry*
  • DNA Helicases / chemistry
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Microscopy, Atomic Force
  • Molecular Motor Proteins / chemistry*
  • Nanotechnology
  • Nucleic Acid Conformation
  • Optical Tweezers
  • Single Molecule Imaging / methods*


  • Molecular Motor Proteins
  • DNA
  • DNA Helicases