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. Jan-Dec 2019;33:2058738419827746.
doi: 10.1177/2058738419827746.

Drug-induced Gingival Hyperplasia: An in Vitro Study Using Amlodipine and Human Gingival Fibroblasts

Free PMC article

Drug-induced Gingival Hyperplasia: An in Vitro Study Using Amlodipine and Human Gingival Fibroblasts

Dorina Lauritano et al. Int J Immunopathol Pharmacol. .
Free PMC article


Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast's function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 (CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.

Keywords: amlodipine; fibroblast; gene expression; gingival overgrowth.

Conflict of interest statement

Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.


Figure 1.
Figure 1.
Amplification plot curves of the 29 genes belonging to the “Inflammatory Cytokines and Receptors” pathway analyzed using real-time PCR.
Figure 2.
Figure 2.
Gene expression profile of fibroblasts treated with amlodipine 1000 ng/mL.

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