Inheritance of pre-emergent metribuzin tolerance and putative gene discovery through high-throughput SNP array in wheat (Triticum aestivum L.)

Roopali Bhoite; Ping Si; Hui Liu; Ling Xu; Kadambot H M Siddique; Guijun Yan

doi:10.1186/s12870-019-2070-x

Inheritance of pre-emergent metribuzin tolerance and putative gene discovery through high-throughput SNP array in wheat (Triticum aestivum L.)

BMC Plant Biol. 2019 Oct 29;19(1):457. doi: 10.1186/s12870-019-2070-x.

Authors

Roopali Bhoite^{1

2}, Ping Si^{1

2}, Hui Liu^{1

2}, Ling Xu^{1

3}, Kadambot H M Siddique², Guijun Yan^{4

5}

Affiliations

¹ UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia.
² The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia.
³ Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
⁴ UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA, 6009, Australia. guijun.yan@uwa.edu.au.
⁵ The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia. guijun.yan@uwa.edu.au.

Abstract

Background: Herbicide tolerance is an important trait that allows effective weed management in wheat crops in dryland farming. Genetic knowledge of metribuzin tolerance in wheat is needed to develop new cultivars for the industry. Here, we investigated gene effects for metribuzin tolerance in nine crosses of wheat by partitioning the means and variances of six basic generations from each cross into their genetic components to assess the gene action governing the inheritance of this trait. Metribuzin tolerance was measured by a visual senescence score 21 days after treatment. The wheat 90 K iSelect SNP genotyping assay was used to identify the distribution of alleles at SNP sites in tolerant and susceptible groups.

Results: The scaling and joint-scaling tests indicated that the inheritance of metribuzin tolerance in wheat was adequately described by the additive-dominance model, with additive gene action the most significant factor for tolerance. The potence ratio for all the crosses ranged between - 1 and + 1 for senescence under metribuzin-treated conditions indicating a semi-dominant gene action in the inheritance of metribuzin tolerance in wheat. The number of segregating genes governing metribuzin tolerance was estimated between 3 and 15. The consistent high heritability range (0.82 to 0.92) in F_5-7 generations of Chuan Mai 25 (tolerant) × Ritchie (susceptible) cross indicated a significant contribution of additive genetic effects to metribuzin tolerance in wheat. Several genes related to photosynthesis (e.g. photosynthesis system II assembly factor YCF48), metabolic detoxification of xenobiotics and cell growth and development (cytochrome P450, glutathione S-transferase, glycosyltransferase, ATP-binding cassette transporters and glutathione peroxidase) were identified on different chromosomes (2A, 2D, 3B, 4A, 4B, 7A, 7B, 7D) governing metribuzin tolerance.

Conclusions: The simple additive-dominance gene effects for metribuzin tolerance will help breeders to select tolerant lines in early generations and the identified genes may guide the development of functional markers for metribuzin tolerance.

Keywords: Candidate genes; Gene effects; Heritability; Inheritance; Metribuzin; Potence ratio; Wheat 90 K iSelect SNP genotyping assay.

MeSH terms

Genetic Association Studies
Herbicide Resistance / genetics*
Herbicides / pharmacology*
Heredity*
Oligonucleotide Array Sequence Analysis
Phenotype
Polymorphism, Single Nucleotide*
Triazines / pharmacology*
Triticum / genetics*

Substances

Herbicides
Triazines
metribuzin