Long non-coding RNA AFAP1-AS1 promotes proliferation and invasion in prostate cancer via targeting miR-512-3p

Kai Wang; Hao Sun; Tao Sun; Hongchen Qu; Qingpeng Xie; Hang Lv; Bin Hu

doi:10.1016/j.gene.2019.144169

Long non-coding RNA AFAP1-AS1 promotes proliferation and invasion in prostate cancer via targeting miR-512-3p

Gene. 2020 Feb 5:726:144169. doi: 10.1016/j.gene.2019.144169. Epub 2019 Nov 26.

Authors

Kai Wang¹, Hao Sun², Tao Sun³, Hongchen Qu¹, Qingpeng Xie¹, Hang Lv¹, Bin Hu⁴

Affiliations

¹ Urology Department, Liaoning Cancer Hospital & Institute, Cancer Hospital of China Medical University, Shengyang City, Liaoning Province 110044, PR China.
² Urology Department, China Medical University, Cancer Hospital of China Medical University, Shengyang City, Liaoning Province 110044, PR China.
³ Urology Department, Dalian Medical University, Dalian Medical University Clinical Oncology College, Shengyang City, Liaoning Province 110044, PR China.
⁴ Urology Department, Liaoning Cancer Hospital & Institute, Cancer Hospital of China Medical University, Shengyang City, Liaoning Province 110044, PR China. Electronic address: tjxgcqff697988@126.com.

PMID: 31669642
DOI: 10.1016/j.gene.2019.144169

Abstract

Background (objective): In the development of tumor therapy, the role of long non-coding RNA actin filagenin 1 antisense RNA 1 (1ncRNA AFAP1-AS1) is quite significant, but the actual role of AFAP1-AS1 in the treatment of prostate cancer has not been determined. In view of this, the author took AFAP1-AS1 as the research object to design an experimental study, and conducted an in-depth exploration of the pathogenesis of prostate cancer.

Methods: RT-qPCR was used to detect the expression of AFAP1-AS1 and miR-512-3p in prostate cancer tissues and cell lines. Perforation, flow cytometry and CCK-8 were used to detect the effects of cell proliferation, migration and invasion of mir-512-3p and a AFAP1-AS1. And the luciferase reporter gene was used to detect the downstream target gene of AFAP1-AS1, and the expression of CDK4, CDK6 and CCND1 protein was detected by Western blot.

Results: AFAP1-AS1 is highly expressed in prostate cancer tissues and cell lines. The expression level of AFAP1-AS1 is correlated with histological grade and distant metastasis. The overall level of patients with high expression of AFAP1-AS1 is low, and their survival rate is relatively low. Silencing AFAP1-AS1 can significantly increase the proliferation and migration of prostate cancer cells. AFAP1-AS1 silencing induces cell cycle arrest at G0/G1 phase. The downstream target of AFAP1-AS1 was mir-512-3p. The role of AFAP1-AS1 in the progression of prostate cancer cells was mediated by mir-512-3p.

Conclusion: AFAP1-AS1 regulates miR-512-3p, so as to realize the regulation effect on the proliferation, invasion and migration of prostate cancer cells, and thereby promote the occurrence and development of prostate cancer, so as to provide the corresponding program for the treatment of prostate cancer. Abberivation: ADPC, androgen-dependent prostate cancer; CRPC, castrated prostate cancer; RNA1 AFAP1-Asl, Actin fiber-associated protein 1-anti-RNA1; miRNAs, MicroRNAs.

Keywords: AFAP1-AS1; Cell cycle; Proliferation; Prostate cancer; miR-512-3p.

MeSH terms

Cell Cycle Checkpoints / genetics
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics*
G1 Phase / genetics
Gene Expression Regulation, Neoplastic / genetics
Humans
Male
MicroRNAs / genetics*
Neoplasm Invasiveness / genetics*
Prostatic Neoplasms / genetics*
RNA, Long Noncoding / genetics*
Resting Phase, Cell Cycle / genetics
Survival Rate

Substances

AFAP1-AS1 long noncoding RNA, human
MIRN512 microRNA, human
MicroRNAs
RNA, Long Noncoding