Neuroprotective effect of biosynthesised gold nanoparticles synthesised from root extract of Paeonia moutan against Parkinson disease - In vitro &In vivo model

J Photochem Photobiol B. 2019 Nov:200:111635. doi: 10.1016/j.jphotobiol.2019.111635. Epub 2019 Oct 16.

Abstract

Parkinson disease is one of the most common neurological movement disorders affecting geriatric population. Biosynthesized gold nanoparticles are the ideal alternatives spotlighted by many researchers to treat various diseases. In the present study we synthesized gold nanoparticles using the root extract of Paeonia mountan, woody trees which are used in traditional Chinese medicine to be prescribed for diverse diseases. The synthesis of gold nanoparticles was confirmed with UV-Vis spectroscopic analysis and characterized using FTIR, HR-TEM, EDAX and XRD analysis. The cytotoxicity property of synthesized gold nanoparticles was assessed using MTT assay in the murine microglial BV2 cells. The neuroprotective effect of synthesized gold nanoparticles in inflammatory agent lipopolysaccharides triggered murine microglial BV2 cells was evaluated using nitric oxide, prostaglandin E2 and inflammatory cytokines assays such as IL-6&IL-1β. Further to confirm in vivo effect of synthesized nanoparticles, the nanoparticles were treated to Parkinson induced C57BL/6 mice. Behavioral, biochemical and molecular analysis were performed to estimate the potency of synthesized gold nanoparticles against the Parkinson induction in mice model. Our characterization results prove the gold nanoparticles synthesized using Paeonia mountan fulfills the requirement of ideal nanodrug and it potentially inhibited the inflammation in in vitro murine microglial BV2. The results of in vivo experiments authentically confirm gold nanoparticles synthesized using Paeonia mountan alleviates the neuroinflammation and improves the motor coordination in Parkinson induced mice.

Keywords: BV2 cells; Cox-2; LPS; Parkinson disease; ROS; TH; TNF-α.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use
  • Antioxidants / chemistry
  • Cell Line
  • Cell Survival / drug effects
  • Cytokines / metabolism
  • Gold / chemistry*
  • Green Chemistry Technology
  • Lipopolysaccharides / pharmacology
  • Male
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / therapeutic use
  • Metal Nanoparticles / toxicity
  • Mice
  • Mice, Inbred C57BL
  • Microglia / cytology
  • Microglia / drug effects
  • Microglia / metabolism
  • Neuroprotective Agents / chemistry*
  • Neuroprotective Agents / pharmacology
  • Neuroprotective Agents / therapeutic use
  • Nitric Oxide / metabolism
  • Paeonia / chemistry*
  • Paeonia / metabolism
  • Parkinson Disease / drug therapy
  • Parkinson Disease / pathology
  • Parkinson Disease / veterinary
  • Plant Extracts / chemistry*
  • Plant Roots / chemistry
  • Plant Roots / metabolism

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Cytokines
  • Lipopolysaccharides
  • Neuroprotective Agents
  • Plant Extracts
  • Nitric Oxide
  • Gold