New AMP-forming acid:CoA ligases from Streptomyces lividans, some of which are posttranslationally regulated by reversible lysine acetylation

Mol Microbiol. 2020 Jan;113(1):253-269. doi: 10.1111/mmi.14414. Epub 2019 Nov 28.


In nature, organic acids are a commonly used source of carbon and energy. Many bacteria use AMP-forming acid:CoA ligases to convert organic acids into their corresponding acyl-CoA derivatives, which can then enter metabolism. The soil environment contains a broad diversity of organic acids, so it is not surprising that bacteria such as Streptomyces lividans can activate many of the available organic acids. Our group has shown that the activity of many acid:CoA ligases is posttranslationally controlled by acylation of an active-site lysine. In some cases, the modification is reversed by deacylases of different types. We identified eight new acid:CoA ligases in S. lividans TK24. Here, we report the range of organic acids that each of these enzymes can activate, and determined that two of the newly identified CoA ligases were under NAD+ -dependent sirtuin deacylase reversible lysine (de)acetylation control, four were not acetylated by two acetyltransferases used in this work, and two were acetylated but not deacetylated by sirtuin. This work provides insights into the broad organic-acid metabolic capabilities of S. lividans, and sheds light into the control of the activities of CoA ligases involved in the activation of organic acids in this bacterium.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acetylation
  • Acyl Coenzyme A / metabolism*
  • Bacterial Proteins / metabolism*
  • Catalytic Domain
  • Coenzyme A Ligases / metabolism*
  • Streptomyces lividans / enzymology*


  • Acyl Coenzyme A
  • Bacterial Proteins
  • Coenzyme A Ligases