Cardiovascular effects of leukotoxin (9, 10-epoxy-12-octadecenoate) and free fatty acids in dogs

Cardiovasc Res. 1988 Mar;22(3):213-8. doi: 10.1093/cvr/22.3.213.


Leukotoxin (9,10-epoxy-12-octadecenoate) biosynthesised from linoleate by neutrophils is highly toxic to cellular function. Its cardiovascular effects were studied in dogs together with the effects of various fatty acids. Aortic flow, left ventricular peak dP.dt-1, and aortic pressure were measured in 60 anaesthetised dogs, which were divided into 10 groups of six animals each--namely, control group (10 ml of physiological saline), three leukotoxin groups (5, 10, and 50, two linoleic acid groups (10 and 50, two oleic acid groups (10 and 50, and two stearic acid groups (10 and 50 Leukotoxin injected intravenously depressed cardiac function in a dose dependent manner. Administration of leukotoxin 5 showed no significant cardiotoxic effect. However, 10 of leukotoxin significantly decreased aortic flow from 0.74(0.04) to 0.40(0.07) litre.min-1 (mean(SEM], left ventricular peak dP.dt-1 from 2040(205) to 1140(217) mmHg.s-1, and aortic pressure from 106(7.1)/67(6.3) to 75(9.2)/48(6.5) mmHg 5 min after injection. Dogs given leukotoxin 50 showed more pronounced cardiodepressive effects; aortic flow was decreased to 0.19(0.06), left ventricular dP.dt-1 to 560(134), and aortic pressure to 72(15.1)/41(10.6) 5 min after injection. All dogs in this group were dead within 45 min. Administration of 10 of linoleic acid, oleic acid, or stearic acid caused no significant haemodynamic changes. Administration of linoleic acid 50 had cardiotoxic effects, but the effect was less than that of leukotoxin. Since leukotoxin appears to be a potent cardiodepressive agent it may be an important factor in the development of heart failure observed in patients with severe burns.

MeSH terms

  • Animals
  • Aorta / physiology
  • Blood Flow Velocity / drug effects
  • Blood Pressure / drug effects
  • Burns / metabolism
  • Cardiovascular System / drug effects*
  • Coronary Circulation / drug effects
  • Dogs
  • Exotoxins / pharmacology*
  • Fatty Acids, Nonesterified / pharmacology*
  • Hemodynamics / drug effects*
  • Time Factors
  • Ventricular Function


  • Exotoxins
  • Fatty Acids, Nonesterified
  • leukotoxin