A new HPTLC platformed luminescent biosensor system for facile screening of captan residue in fruits

Yisheng Chen; Caihong Huang; Bernhard Hellmann; Zhengyu Jin; Xueming Xu; Guosheng Xiao

doi:10.1016/j.foodchem.2019.125691

A new HPTLC platformed luminescent biosensor system for facile screening of captan residue in fruits

Food Chem. 2020 Mar 30:309:125691. doi: 10.1016/j.foodchem.2019.125691. Epub 2019 Oct 19.

Authors

Yisheng Chen¹, Caihong Huang², Bernhard Hellmann³, Zhengyu Jin⁴, Xueming Xu⁵, Guosheng Xiao⁶

Affiliations

¹ The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China. Electronic address: chen.yisheng@jiangnan.edu.cn.
² School of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
³ Institute of Nutritional Science, Justus Liebig University Giessen, Wilhelmstraße 20, 35392 Giessen, Germany.
⁴ The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
⁵ The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China. Electronic address: xmxu@jiangnan.edu.cn.
⁶ College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou 404100, China.

PMID: 31679853
DOI: 10.1016/j.foodchem.2019.125691

Abstract

This study presented a HPTLC platformed luminescent biosensor system for screening captan residue. First, the potential bio-effects of layers materials on the detectability of a luminescent bacteria Photobacterium phosphoreum (ATCC 11040) as the sensor cell were assessed. From comparison, it was noteworthy that the combination of sensor cells with normal silica gel layer exclusively gave outstanding detectability (<10 ng/zone). On this basis, HPTLC mediated separation and biosensing was further optimized. Then, the obtained graphic results were digitally quantified via software processing, offering satisfactory selectivity, linearity (R² = 0.9901 within 10-80 ng/zone) and sensitivity (0.5 mg/kg against MRLs ≥ 6 mg/kg). Additionally, the performance of the established method was validated with different fruits (recover rates 75-96%, RSD < 11.8%). Meanwhile, it was demonstrated that detectability of this hybrid system would be tuneable by altering the combination of bacteria strains and layer materials, which was meaningful to strengthen the usability of microbial biosensors.

Keywords: Captan screening; Fruits; High performance thin-layer chromatography; Luminescent biosensor.

MeSH terms

Biosensing Techniques / methods*
Captan / analysis*
Chromatography, Thin Layer
Fruit / chemistry
Fruit / metabolism
Fungicides, Industrial / analysis*
Malus / chemistry
Malus / metabolism
Photobacterium / chemistry
Photobacterium / isolation & purification
Silica Gel / chemistry

Substances

Fungicides, Industrial
Silica Gel
Captan