Functional Connectivity Patterns and the Role of 5-HTTLPR Polymorphism on Network Architecture in Female Patients With Anorexia Nervosa

Enrico Collantoni; Paolo Meneguzzo; Marco Solmi; Elena Tenconi; Renzo Manara; Angela Favaro

doi:10.3389/fnins.2019.01056

Functional Connectivity Patterns and the Role of 5-HTTLPR Polymorphism on Network Architecture in Female Patients With Anorexia Nervosa

Front Neurosci. 2019 Oct 14:13:1056. doi: 10.3389/fnins.2019.01056. eCollection 2019.

Authors

Enrico Collantoni¹, Paolo Meneguzzo¹, Marco Solmi^{1

2}, Elena Tenconi^{1

2}, Renzo Manara³, Angela Favaro^{1

2}

Affiliations

¹ Department of Neurosciences, University of Padua, Padua, Italy.
² Padova Neuroscience Center, University of Padua, Padua, Italy.
³ Radiology Unit, Department of Medicine and Surgery, Neuroscience Section, University of Salerno, Salerno, Italy.

Abstract

Introduction: Recent neuroimaging studies suggest that anorexia nervosa (AN) symptoms emerge from failures in the relationships between spatially distributed networks that support different cognitive, emotional, and somatosensory functions. The 5-HTTLPR genotype has been shown to modulate all these abilities in AN, as well as the connectivity patterns between brain regions that support their functioning. This study aims at exploring the presence of any difference in functional connectome properties between AN patients and healthy controls (HC) by means of graph theory tools. The effect of 5-HTTLPR genotype on regional and global network characteristics in AN and HC was also explored.

Methods: A sample of 74 subjects (38 HC, 36 AN) underwent a resting state functional magnetic resonance imaging and was genotyped for 5-HTTLPR polymorphism. Comparisons of network properties were made between the AN and HC groups and, within each group, between 5-HTTLPR carriers of low-functioning alleles and carriers of the long-long genotype.

Results: Patients with AN displayed lower network clustering than HC (p = 0.04 at Mann-Whitney U test). Based on both degree and betweenness, a different distribution of network hubs emerged in the two groups. In particular, the anterior part of the anterior cingulate cortex was a hub only in the patient group. A correlation emerged between differences in brain volumes between patients and HC and differences in degree values of basal ganglia, nodes in the insula, and those in the parietal cortex. Carriers of the short allele of the 5-HTTLPR polymorphism were characterized by lower small-world properties (p = 0.027) and modularity (p = 0.031) in the patient group, and a trend toward higher modularity (p = 0.033) and small-world values (p = 0.123) in the HC group.

Discussion: Patients with AN showed differences in hubs distribution, providing evidence of the presence of a different functional architectural backbone in this group. Since some correlation emerged between different degree values of nodes and differences in volumes, further longitudinal studies are warranted to better understand the role of malnutrition on brain network architecture. The opposite effects of 5-HTTLPR polymorphism on global network characteristics in the two groups suggest an interaction of the short allele and malnutrition in modulating brain network properties.

Keywords: 5-HTTLPR; anorexia nervosa; eating disorders; fMRI; graph theory; neuroimaging; resting state.