Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes

Stem Cell Res. 2019 Dec;41:101590. doi: 10.1016/j.scr.2019.101590. Epub 2019 Oct 15.

Abstract

Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, such as polycistronic KLF4-OCT3/4-SOX2, cMYC, and KLF4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Line
  • Cellular Reprogramming Techniques*
  • Epidermis / metabolism*
  • Female
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism*
  • Keratinocytes / cytology
  • Keratinocytes / metabolism*