Genetic diversity of Paramecium species on the basis of multiple loci analysis and ITS secondary structure models

Fareeda Tasneem; Farah R Shakoori; Muhammad Ilyas; Naveed Shahzad; Alexey Potekhin; Abdul R Shakoori

doi:10.1002/jcb.29546

Genetic diversity of Paramecium species on the basis of multiple loci analysis and ITS secondary structure models

J Cell Biochem. 2020 Aug;121(8-9):3837-3853. doi: 10.1002/jcb.29546. Epub 2019 Nov 6.

Authors

Fareeda Tasneem¹, Farah R Shakoori¹, Muhammad Ilyas², Naveed Shahzad², Alexey Potekhin³, Abdul R Shakoori^{1

2}

Affiliations

¹ Department of Zoology, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
² School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
³ Department of Microbiology, Faculty of Biology, Saint Petersburg State University, Saint Petersburg, Russia.

PMID: 31692070
DOI: 10.1002/jcb.29546

Abstract

Among ciliates, Paramecium has become a privileged model for the study of "species problem" particularly in the case of the "Paramecium aurelia complex" that has been intensely investigated. Despite extensive studies, the taxonomy of Paramecium is still challenging. The major problem is an uneven sampling of Paramecium with relatively few representatives of each species. To investigate species from the less discovered region (Pakistan), 10 isolates of Paramecium species including a standing-alone FT8 strain previously isolated by some of us were subjected to molecular characterization. Fragments of 18S recombinant DNA (rDNA), ITS1-5.8S-ITS2-5'LSU rDNA, cytochrome c oxidase subunit II, and hsp70 genes were used as molecular markers for phylogenetic analysis of particular isolates. The nucleotide sequences of polymerase chain reaction products of all markers were compared with the available sequences of relevant markers of other Paramecium species from GenBank. Phylogenetic trees based on all molecular markers showed that all the nine strains had a very close relationship with Paramecium primaurelia except for the FT8 strain. FT8 consistently showed its unique position in comparison to all other species in the phylogenetic trees. Available sequences of internal transcribed spacer 1 (ITS1) and ITS2 and some other ciliate sequences from GenBank were used for the construction of secondary models. Two highly conserved helices supported by compensatory base changes among all ciliates of ITS2 secondary structures were found similar to other eukaryotes. Therefore, the most conserved 120 to 180 base pairs regions were identified for their comparative studies. We found that out of the three helices in ITS1 structure, helix B was more conserved in Paramecium species. Despite various substitutions in the primary sequence, it was observed that secondary structures of ITS1 and ITS2 could be helpful in interpreting the phylogenetic relationships both at species as well as at generic level.

Keywords: 18S rDNA; DNA barcoding; Hsp70; ITS1 and ITS2 secondary structures; ciliates; internal transcribed spacer regions; mitochondrial cytochrome c oxidase subunit II.