Comparison of two DNA extraction methods from larvae, pupae, and adults of Aedes aegypti

Josué M de la Cruz-Ramos; Luis M Hernández-Triana; Cristina García-De la Peña; Vicente H González-Álvarez; James Weger-Lucarelli; Quetzaly Karmy Siller-Rodríguez; Francisco J Sánchez Rámos; Américo D Rodríguez; Aldo I Ortega-Morales

doi:10.1016/j.heliyon.2019.e02660

Comparison of two DNA extraction methods from larvae, pupae, and adults of Aedes aegypti

Heliyon. 2019 Oct 16;5(10):e02660. doi: 10.1016/j.heliyon.2019.e02660. eCollection 2019 Oct.

Affiliations

¹ Universidad Autónoma Agraria Antonio Narro Unidad Laguna, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico.
² Unidad de Investigaciones Entomológicas y de Bioensayos del estado de Durango, Periférico y carretera Santa Fe, 27084, Torreón, Coahuila, Mexico.
³ Animal and Plant Health Agency, Virology Department, Wildlife Zoonoses and Vector Borne Diseases Research Group, Woodham Lane, New Haw, Addlestone, Surrey, KT153NB, UK.
⁴ Facultad de Ciencias Biológicas, Universidad Juárez del Estado de Durango, Av. Universidad, 35010, Gómez Palacio, Durango, Mexico.
⁵ Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciajinicuilapa, Guerrero, Mexico.
⁶ Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
⁷ Centro Regional de Investigación en Salud Publica, Instituto Nacional de Salud Publica, Tapachula, Chiapas, Mexico.

Abstract

Mosquitoes are the most important arthropods from the point of view of public health, due to the fact that they can transmit a large number of pathogens which can cause diseases to humans and animals. Aedes aegypti (L.) is one of the most important vector species in the world, since it can transmit numerous pathogens such as dengue, Zika, and chikungunya. Therefore, studies involving the molecular aspects of this and other mosquitoes species are currently increasing. In this report, we describe the comparison between two DNA extraction techniques, Chelex and cetyltrimethylammonium bromide (CTAB), for carrying out DNA extraction in larvae, pupae and adult female of Ae. aegypti. The Chelex technique was superior in the amount and purity of DNA as compared to the CTAB technique in the three life stages we tested.

Keywords: Aedes aegypti; Biochemistry; CTAB; Chelex; Comparison; DNA extraction; Genetics; Microbiology; Molecular biology; Public health.