Validation of a questionnaire about knowledge and perception of biological risk among biomedical students of Sapienza University of Rome

Clin Ter. 2019 Nov-Dec;170(6):e430-e434. doi: 10.7417/CT.2019.2172.


Background adn aim: Healthcare workers and Biomedical students are continuously exposed to biological risk in their clinical practice. The objective of this study was to evaluate the validity and reliability of an Italian questionnaire on the knowledge and perception of biological risk in Biomedical students at the beginning of their professional training.

Materials and methods: An electronic questionnaire was administered to students attending the second semester of the first year of Biomedical Courses at Sapienza University of Rome. The questionnaire consists of 40 questions divided into five sections collecting sociodemographic data, health status and level of knowledge and perception of biological risk. The statistical analysis was performed with Statistical Package for Social Sciences (SPSS) version 25.

Results: A total of 309 individuals answered the online questionnaire, with a response rate of 83.5%. The analysis of internal consistency was performed by two dichotomous variables that measured the knowledge level on hygiene behaviour and gloves use. The analysis showed a standardized Cronbach's alpha equal to 0.765, corresponding to a good reliability. A better reliability was found out among physiotherapy and medical students, with a Cronbach's alpha equal to 0.944 and 0.881, respectively. Regarding vaccines, 97.7% of the sample was given a Hepatitis B vaccination and 98.7% of students consider vaccinations essential for healthcare workers.

Conclusions: Results of Cronbach's alpha showed a good reliability of the questionnaire. First-year Biomedical students may be exposed to occupational biological risk mainly because of their inexperience. A training educational path should be implemented in order to acquire competences, knowledge, attitudes and practical skills, correct behaviors and a personal and professional responsibility.

Keywords: Biological risk; Knowledge; Occupational exposure; Perception; Students; University.

Publication types

  • Validation Study

MeSH terms

  • Attitude
  • Female
  • Health Knowledge, Attitudes, Practice*
  • Humans
  • Italy
  • Male
  • Occupational Diseases*
  • Reproducibility of Results
  • Risk Assessment
  • Students, Medical*
  • Surveys and Questionnaires*
  • Young Adult