NIR-II Fluorescence Endoscopy for Targeted Imaging of Colorectal Cancer

Adv Healthc Mater. 2019 Dec;8(23):e1900974. doi: 10.1002/adhm.201900974. Epub 2019 Nov 7.

Abstract

Endoscopy is a clinical gold standard to exam the interior of a hollow organ or body cavity. For the first of time, this study presents the design and construction of a fluorescent endoscopic system that harnesses the power of the second near-infrared window II (NIR-II) fluorescence imaging. An NIR-II fluorescent molecular probe, indocyanine green (ICG) conjugated bevacizumab (Bev-ICG) that targets vascular endothelial growth factor (VEGF), is successfully synthesized and evaluated along with the NIR-II endoscopy imaging system. Simultaneous NIR-II fluorescence and white-light (WL) imaging of VEGF is validated in an orthotopic rat colorectal cancer model. This NIR-II endoscopy system is a generalizable design, and it is compatible with the most of current clinic endoscopies. Similar hardware upgrades are expected to greatly promote the application of NIR-II fluorescent imaging in the clinic.

Keywords: colorectal cancer; endoscopy; fluorescence; molecular imaging; the second near-infrared window.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bevacizumab / chemistry
  • Blotting, Western
  • Colorectal Neoplasms / diagnostic imaging*
  • Electrophoresis, Polyacrylamide Gel
  • Endoscopy / methods*
  • Indocyanine Green / chemistry
  • Microscopy, Fluorescence
  • Rats
  • Spectroscopy, Near-Infrared
  • Vascular Endothelial Growth Factor A / chemistry

Substances

  • Vascular Endothelial Growth Factor A
  • Bevacizumab
  • Indocyanine Green