Orosomucoid (alpha-1 acid glycoprotein) phenotyping by use of immobilized pH gradients with 8 M urea and immunoblotting. A new variant encountered in a population study

Hum Genet. 1988 Oct;80(2):183-5. doi: 10.1007/BF00702865.

Abstract

Orosomucoid (ORM) phenotyping has been performed on 329 unrelated Swiss subjects, using immobilized pH gradients with 8 M urea and 2% v/v 2-mercaptoethanol followed by immunoblotting. After desialylation the band patterns of ORM confirmed that the polymorphism of the structural locus ORM1 is controlled by three codominant autosomal alleles (ORM1*F1, ORM1*S and ORM1*F2). One rare and one new allele were detected. The rare variant, tentatively assigned to the second structural locus ORM2, is observed in a cathodal position and named ORM2 B1. The new variant, tentatively assigned to the first structural locus ORM1, is observed in a region located between ORM1 S and ORM1 F2, and named ORM1 F3. Moreover, the pI values of the ORM variants have been measured accurately with Immobiline Dry Plates (LKB): they were found to be within the pH range 4.93-5.14.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genes
  • Genes, Dominant
  • Humans
  • Hydrogen-Ion Concentration
  • Immunoblotting
  • Orosomucoid / genetics*
  • Phenotype
  • Urea

Substances

  • Orosomucoid
  • Urea