The effect of the artificial sweeteners on glucose metabolism in healthy adults: a randomized, double-blinded, crossover clinical trial

Appl Physiol Nutr Metab. 2020 Jun;45(6):606-612. doi: 10.1139/apnm-2019-0359. Epub 2019 Nov 7.


This study aimed to determine the effect of pure forms of sucralose and aspartame, in doses reflective of common consumption, on glucose metabolism. Healthy participants consumed pure forms of a non-nutritive sweetener (NNS) that were mixed with water and standardized to doses of 14% (0.425 g) of the acceptable daily intake (ADI) for aspartame and 20% (0.136 g) of the ADI for sucralose every day for 2 weeks. Blood samples were collected and analyzed for glucose, insulin, active glucagon-like peptide-1 (GLP-1), and leptin. Seventeen participants (10 females and 7 males; age, 24 ± 6.8 years; body mass index, 22.9 ± 2.5 kg/m2) participated in the study. The total area under the curve values of glucose, insulin, active GLP-1 and leptin were similar for the aspartame and sucralose treatment groups compared with the baseline values in healthy participants. There was no change in insulin sensitivity after NNS treatment compared with the baseline values. These findings suggest that daily repeated consumption of pure sucralose or aspartame for 2 weeks had no effect on glucose metabolism among normoglycaemic adults. However, these results need to be tested in studies with longer durations. Novelty Daily consumption of pure aspartame or sucralose for 2 weeks had no effect on glucose metabolism. Daily consumption of pure aspartame or sucralose for 2 weeks had no effect on insulin sensitivity among healthy adults.

Keywords: GLP-1 actif; active GLP-1; aspartame; glucose; glucose metabolism; insulin; insuline; leptin; leptine; métabolisme du glucose; non-nutritive sweetener; protocol; protocole; sucralose; édulcorant non nutritif.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adolescent
  • Adult
  • Aspartame / pharmacology
  • Blood Glucose / analysis
  • Blood Glucose / metabolism*
  • Carbohydrate Metabolism / drug effects*
  • Cross-Over Studies
  • Double-Blind Method
  • Female
  • Humans
  • Insulin Resistance / physiology
  • Male
  • Sucrose / analogs & derivatives
  • Sucrose / pharmacology
  • Sweetening Agents / pharmacology*
  • Young Adult


  • Blood Glucose
  • Sweetening Agents
  • Sucrose
  • trichlorosucrose
  • Aspartame