Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography

Jiaqin Tang; Xudong Zhang; Tao Chen; Ying Wang; Yifeng Li

doi:10.1016/j.pep.2019.105529

Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography

Protein Expr Purif. 2020 Mar:167:105529. doi: 10.1016/j.pep.2019.105529. Epub 2019 Nov 4.

Authors

Jiaqin Tang¹, Xudong Zhang¹, Tao Chen¹, Ying Wang¹, Yifeng Li²

Affiliations

¹ Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
² Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China. Electronic address: li_yifeng@wuxiapptec.com.

PMID: 31698035
DOI: 10.1016/j.pep.2019.105529

Abstract

During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Among them, half antibody and homodimer are found with high frequency. In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. This makes MMC ImpRes a powerful tool for bsAb purification.

Keywords: Antibody aggregates; Bispecific antibody (bsAb); Capto MMC ImpRes; Half antibody; Hole-hole homodimer; Mixed-mode chromatography.

MeSH terms

Antibodies, Bispecific / analysis
Antibodies, Bispecific / biosynthesis
Antibodies, Bispecific / isolation & purification*
Chromatography / methods*
Chromatography, Gel
Chromatography, High Pressure Liquid
Dimerization
Humans
Immunoglobulin G / analysis
Immunoglobulin G / biosynthesis
Immunoglobulin G / isolation & purification
Protein Engineering / methods

Substances

Antibodies, Bispecific
Immunoglobulin G