An integration-free iPSC line, ICCSICi007-A, derived from a female Alzheimer's disease patient with the APOE-ε4/ε4 alleles

Stem Cell Res. 2019 Dec;41:101588. doi: 10.1016/j.scr.2019.101588. Epub 2019 Oct 8.

Abstract

The epsilon4 (ε4) allele of the APOE gene, which encodes the apolipoprotein E4 (ApoE4), is the strongest genetic risk factor known for late-onset Alzheimer´s disease (LOAD). Here, we present the characterization of an iPSC line generated from dermal fibroblasts of a female AD patient using Sendai viral vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The iPSCs maintained the original genotype, a normal karyotype, were free from Sendai viral vectors and reprogramming factors, presented a normal morphology, expressed endogenous pluripotency markers, and could be differentiated into ectodermal, mesodermal and endodermal cells, confirming its pluripotency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Alzheimer Disease* / genetics
  • Alzheimer Disease* / metabolism
  • Alzheimer Disease* / pathology
  • Apolipoproteins E* / genetics
  • Apolipoproteins E* / metabolism
  • Cell Line
  • Cellular Reprogramming Techniques
  • Dermis* / metabolism
  • Dermis* / pathology
  • Female
  • Fibroblasts* / metabolism
  • Fibroblasts* / pathology
  • Humans
  • Induced Pluripotent Stem Cells*
  • Kruppel-Like Factor 4

Substances

  • ApoE protein, human
  • Apolipoproteins E
  • KLF4 protein, human
  • Kruppel-Like Factor 4