Lead-induced oxidative stress triggers root cell wall remodeling and increases lead absorption through esterification of cell wall polysaccharide

Abstract

Tall fescue (Festuca arundinacea Schreb) shows remarkable tolerance to lead (Pb), but the mechanisms involved in metal tolerance are not yet well understood. Here, tall fescue were firstly cultivated hydroponically with Pb²⁺ (0, 50, 200 and 1000 mg/L) for 14 days. The results showed that remodeling of root architecture plays important roles in tolerance of tall fescue to Pb²⁺ stress. Increased cell wall (CW) components contribute to restrict high amount of Pb²⁺ in roots. Additionally, the uronic acid contents of pectin, hemicellulose 1 (HC1) and hemicellulose 2 (HC2) increased under Pb²⁺ stress. We further observed that tall fescue cultivated with H₂O₂ showed similar remodeling of root architecture as Pb²⁺ treatment. Furthermore, pectin, HC1 and HC2 fractions were sequentially extracted from 0 and 10 mM H₂O₂ treated roots, and Pb²⁺ adsorption capacity and contents of carboxyl groups of pectin and HC2 fractions were steadily increased under H₂O₂ treatment in vitro. Our results suggest that degrees of esterification of pectin and HC2 are regulated by H₂O₂. High amount of low-esterified pectin and HC2 offer more carboxyl groups, provide more Pb²⁺ binding sites, and restrict more Pb²⁺ in the CW, which may enhance tolerance of tall fescue to Pb²⁺ stress.

Keywords: Carboxyl groups; Lead; Reactive oxygen species; Root architecture; Tall fescue.