LncRNA NEAT1 polymorphisms and lung cancer susceptibility in a Chinese Northeast Han Population: A case-control study

Shengli Wang; Zhigang Cui; Hang Li; Juan Li; Xiaoting Lv; Zitai Yang; Min Gao; Yanhong Bi; Ziwei Zhang; Baosen Zhou; Zhihua Yin

doi:10.1016/j.prp.2019.152723

LncRNA NEAT1 polymorphisms and lung cancer susceptibility in a Chinese Northeast Han Population: A case-control study

Pathol Res Pract. 2019 Dec;215(12):152723. doi: 10.1016/j.prp.2019.152723. Epub 2019 Oct 31.

Authors

Shengli Wang¹, Zhigang Cui², Hang Li³, Juan Li⁴, Xiaoting Lv⁵, Zitai Yang⁶, Min Gao⁷, Yanhong Bi⁸, Ziwei Zhang⁹, Baosen Zhou¹⁰, Zhihua Yin¹¹

Affiliations

¹ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: Wangshengli@cmu.edu.cn.
² School of Nursing, China Medical University, Shenyang 110122, PR China. Electronic address: zgcui@cmu.edu.cn.
³ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: lihangatcmu@126.com.
⁴ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: lijuan@cmu.edu.cn.
⁵ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: cmulxt1992@163.com.
⁶ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: cmuzitaiyang@163.com.
⁷ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: mgao@cmu.edu.cn.
⁸ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: cmuyhbi@126.com.
⁹ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: 1582467076@qq.com.
¹⁰ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: bszhou@cmu.edu.cn.
¹¹ Department of Epidemiology, School of Public Health, China Medical University, Shenyang 110122, PR China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang 110122, PR China. Electronic address: zhyin@cmu.edu.cn.

PMID: 31704150
DOI: 10.1016/j.prp.2019.152723

Abstract

Background: Long non-coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) is a novel lncRNA localized specifically to nuclear paraspeckles. The study analyses the association between NEAT1 genetic polymorphisms and the susceptibility of lung cancer in a Chinese Northeast Population.

Methods: The NEAT1 rs512715 and rs2239895 genetic polymorphisms were genotyped in 462 lung cancer cases and 559 controls by a Real-Time Polymerase Chain Reaction (PCR) with the TaqMan discrimination assay.

Results: Our study found that the polymorphisms of two SNPs increased or decreased the risk of lung cancer were not obvious, but statistical significance in non-small cell lung cancer and lung squamous cell carcinoma can be observed. Compared with homozygous CC genotype carriers, the GC genotype of rs2239895 was positively related to the risk of lung squamous cell carcinoma (OR 1.805, 95% CI, 1.168-2.789, P = 0.008). Similarly, associations between rs2239895 and lung squamous cell carcinoma risk were found (CC + GC vs. GG, OR 1.668, 95%CI, 1.093-2.545, P = 0.018) in dominant model. In stratified analysis for age, rs2239895 GC genotype was observed to increase the risk of non-small-cell lung cancer compared with CC genotype (OR 1.562, 95%CI, 1.029-2.371, P = 0.036). However, the study showed that negative correlation the lung cancer risk and rs512715 polymorphisms. There was no remarkable relationship between the both additive and multiplicative model about the two SNPs.

Conclusions: The polymorphisms rs2239895 were associated with the risk of lung squamous cell carcinoma. The interaction between the two SNPs and the cigarette smoking was no notable difference.

Keywords: Interaction; Long noncoding RNA; Lung cancer; NEAT1; Single nucleotide polymorphism.

MeSH terms

Adenocarcinoma of Lung / diagnosis
Adenocarcinoma of Lung / ethnology
Adenocarcinoma of Lung / genetics*
Adult
Aged
Asian People / genetics
Biomarkers, Tumor / genetics*
Carcinoma, Non-Small-Cell Lung / diagnosis
Carcinoma, Non-Small-Cell Lung / ethnology
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Squamous Cell / diagnosis
Carcinoma, Squamous Cell / ethnology
Carcinoma, Squamous Cell / genetics*
Case-Control Studies
China / epidemiology
Female
Genetic Association Studies
Genetic Predisposition to Disease
Humans
Lung Neoplasms / diagnosis
Lung Neoplasms / ethnology
Lung Neoplasms / genetics*
Male
Middle Aged
Polymorphism, Single Nucleotide*
RNA, Long Noncoding / genetics*
Risk Assessment
Risk Factors
Smoking / adverse effects
Smoking / ethnology

Substances

Biomarkers, Tumor
NEAT1 long non-coding RNA, human
RNA, Long Noncoding