Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis

J Neuroimmunol. 2020 Jan 15;338:577090. doi: 10.1016/j.jneuroim.2019.577090. Epub 2019 Oct 26.

Abstract

Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS).

Keywords: Inflammation; Leptin; Multiple sclerosis; Oxidative response; Peripheral blood mononuclear cells; mRNA expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Female
  • Humans
  • Leptin / blood
  • Leptin / genetics*
  • Leukocytes, Mononuclear / metabolism*
  • Male
  • Middle Aged
  • Multiple Sclerosis, Relapsing-Remitting / etiology
  • Multiple Sclerosis, Relapsing-Remitting / metabolism*
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / blood
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / genetics*
  • RNA, Messenger / analysis
  • Reactive Oxygen Species / metabolism
  • Receptors, Leptin / blood
  • Receptors, Leptin / genetics*
  • Tumor Necrosis Factor-alpha / genetics
  • Young Adult

Substances

  • Leptin
  • PPARGC1A protein, human
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • RNA, Messenger
  • Reactive Oxygen Species
  • Receptors, Leptin
  • Tumor Necrosis Factor-alpha