Optical voltage imaging in neurons: moving from technology development to practical tool

Nat Rev Neurosci. 2019 Dec;20(12):719-727. doi: 10.1038/s41583-019-0231-4. Epub 2019 Nov 8.


A central goal in neuroscience is to determine how the brain's neuronal circuits generate perception, cognition and emotions and how these lead to appropriate behavioural actions. A methodological platform based on genetically encoded voltage indicators (GEVIs) that enables the monitoring of large-scale circuit dynamics has brought us closer to this ambitious goal. This Review provides an update on the current state of the art and the prospects of emerging optical GEVI imaging technologies.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Biomedical Technology / methods
  • Biomedical Technology / trends*
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescence Resonance Energy Transfer / trends*
  • Humans
  • Neurons / chemistry*
  • Neurons / physiology
  • Optogenetics / methods
  • Optogenetics / trends*
  • Voltage-Sensitive Dye Imaging / methods
  • Voltage-Sensitive Dye Imaging / trends*