Abstract
The thromboxane A2 antagonist, ONO-3708, completely inhibited the increase in cytosolic free Ca2+ in human platelets during activation with collagen. Half-maximal Ca2+ release and influx required about 3 and 4 nM STA2, a stable thromboxane A2 mimetic, respectively. However, half maximal activation of phospholipase C required about 18 nM STA2. This suggests that thromboxane A2 directly causes Ca2+ mobilization without further activation of phospholipase C during activation of human platelets with collagen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blood Platelets / metabolism*
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Calcium / metabolism*
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Collagen / pharmacology*
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Cytosol / metabolism*
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Enzyme Activation
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Humans
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In Vitro Techniques
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Platelet Activating Factor / pharmacology*
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Thromboxane A2 / analogs & derivatives
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Thromboxane A2 / antagonists & inhibitors
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Thromboxane A2 / pharmacology
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Thromboxane A2 / physiology*
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Type C Phospholipases / metabolism
Substances
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Platelet Activating Factor
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Thromboxane A2
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ONO 3708
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Collagen
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Type C Phospholipases
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Calcium