An induced pluripotent stem cells line (SDQLCHi014-A) derived from urine cells of a patient with ASD and hyperactivity carrying a 303 kb de novo deletion at chr3p26.1 implicating GRM7 gene

Xiaomeng Yang; Yong Liu; Tiancheng Zhou; Haiyan Zhang; Rui Dong; Yue Li; Ning Liu; Yi Liu; Zhongtao Gai

doi:10.1016/j.scr.2019.101635

An induced pluripotent stem cells line (SDQLCHi014-A) derived from urine cells of a patient with ASD and hyperactivity carrying a 303 kb de novo deletion at chr3p26.1 implicating GRM7 gene

Stem Cell Res. 2019 Dec:41:101635. doi: 10.1016/j.scr.2019.101635. Epub 2019 Nov 2.

Authors

Xiaomeng Yang¹, Yong Liu², Tiancheng Zhou³, Haiyan Zhang¹, Rui Dong¹, Yue Li¹, Ning Liu¹, Yi Liu⁴, Zhongtao Gai⁵

Affiliations

¹ Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China.
² Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China; Equipment Division, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China.
³ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China.
⁴ Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China. Electronic address: y_liu99@sina.com.
⁵ Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China. Electronic address: gaizhongtao@sina.com.

PMID: 31707215
DOI: 10.1016/j.scr.2019.101635

Abstract

Autism spectrum disorder (ASD) is a childhood-onset neurodevelopmental disorder challenged in social reciprocity and restrictive repetitive behaviors. Here, we generated an induced pluripotent stem cell (iPSC) line SDQLCHi014-A from a patient with ASD and hyperactivity, carrying a 303 kb de novo deletion at chr3p26.1 implicating GRM7 gene by reprogramming urine cells with non-integrating vectors. SDQLCHi014-A have shown full pluripotency, differentiation capacity and genetic stability. This iPSC line provides a valuable resource to study the molecular mechanisms underlying ASD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Attention Deficit Disorder with Hyperactivity / complications*
Autism Spectrum Disorder / genetics
Autism Spectrum Disorder / pathology*
Autism Spectrum Disorder / urine*
Base Pairing*
Cell Line
Child
Chromosomes, Human, Pair 3 / genetics*
Humans
Induced Pluripotent Stem Cells / pathology*
Receptors, Metabotropic Glutamate / genetics*
Reproducibility of Results
Sequence Deletion / genetics*

Substances

Receptors, Metabotropic Glutamate
metabotropic glutamate receptor 7