Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies

Robert D Schwab; Darel Martínez Bedoya; Tiffany R King; Bruce L Levine; Avery D Posey Jr

doi:10.1007/978-1-0716-0146-4_15

Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies

Methods Mol Biol. 2020:2086:203-211. doi: 10.1007/978-1-0716-0146-4_15.

Authors

Robert D Schwab¹, Darel Martínez Bedoya¹, Tiffany R King¹, Bruce L Levine^{1

2}, Avery D Posey Jr^{3

4

5}

Affiliations

¹ Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
² Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
³ Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. aposey@pennmedicine.upenn.edu.
⁴ Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. aposey@pennmedicine.upenn.edu.
⁵ Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA. aposey@pennmedicine.upenn.edu.

PMID: 31707678
DOI: 10.1007/978-1-0716-0146-4_15

Abstract

Chimeric antigen receptor (CAR) T cell therapies are ex vivo manufactured cellular products that have been useful in the treatment of blood cancers and solid tumors. The quality of the final cellular product is influenced by several amenable factors during the manufacturing process. This review discusses several of the influences on cell product phenotype, including the raw starting material, methods of activation and transduction, and culture supplementation.

Keywords: CAR-T cells; T cell manufacturing.

MeSH terms

Biomarkers
Cell Culture Techniques / methods
Cell Differentiation* / genetics
Cell Differentiation* / immunology
Humans
Immunophenotyping
Immunotherapy, Adoptive* / methods
Lymphocyte Activation / genetics
Lymphocyte Activation / immunology*
Phenotype
Receptors, Antigen, T-Cell / genetics
Receptors, Antigen, T-Cell / metabolism*
Receptors, Chimeric Antigen / genetics
Receptors, Chimeric Antigen / metabolism*
T-Lymphocytes / immunology*
T-Lymphocytes / metabolism*

Substances

Biomarkers
Receptors, Antigen, T-Cell
Receptors, Chimeric Antigen