Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 12 (3), 233-236

Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP-ACP and Tricalcium Phosphate: An In Vitro Study


Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP-ACP and Tricalcium Phosphate: An In Vitro Study

Venu Varma et al. Int J Clin Pediatr Dent.


Aim: The aim of this study is to evaluate the remineralization potential of Clinpro XT varnish containing tricalcium phosphate (TCP) and MI varnish containing casein phosphopeptide (casein phosphopeptide-amorphous calcium phosphate, CPP-ACP).

Materials and methods: Thirty premolar teeth were taken and divided into three groups. Samples were sliced mesiodistally into buccal and lingual halves using a diamond disk bur. The buccal halves of the teeth were used for the study. Artificial caries like lesions were produced and evaluated with Diagnodent. The samples in each group were treated with the respective remineralizing agent (except for the control group) at every 24 hours for 7 days and the surfaces were assessed using Diagnodent to record the values after the remineralization procedure. The Diagnodent values obtained were tabulated and statistically analyzed using one-way ANOVA and Tukey's multiple comparison tests.

Results: There was a significant difference between the values calculated before and after remineralization in all the three groups.

Conclusion: The study findings showed that MI varnish containing CPP-ACP had the highest release of fluoride as compared to the Clinpro fluoride releasing varnish.

Clinical significance: MI varnish is a 5% NaF varnish containing CPP-ACP to give an exceptional fluoride varnish that releases more bioavailable fluoride, calcium, and phosphate, hence, can be used successfully in remineralization of early carious lesions. CPP-ACP can be used in the clinical practice for reversing or arresting the early carious lesions.

How to cite this article: Varma V, Hegde KS, et al. Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP-ACP and Tricalcium Phosphate: An In Vitro Study. Int J Clin Pediatr Dent 2019;12(3):233-236.

Keywords: Clinpro varnish; MI varnish with CPP–ACP; Tooth remineralization.

Conflict of interest statement

Source of support: Nil Conflict of interest: None


Fig. 1
Fig. 1
Teeth divided into three groups
Fig. 2
Fig. 2
Fig. 3
Fig. 3
Checking the values with Diagnodent
Fig. 4
Fig. 4
Comparing three different groups: group I (MI varnish with CPP–ACP) showed better reminarlization than group II (Clinpro XT varnish with durable fluoride-releasing coating) and group III (control group)

Similar articles

See all similar articles


    1. Jayarajan J, Janardhanam P, et al. Efficacy of CPP–ACP and CPP–ACPF on enamel remineralization-an in vitro study using scanning electron microscope and DIAGNOdent®. Indian J Dent Res. 2011 Jan 1;22(1):77. doi: 10.4103/0970-9290.80001. DOI: - DOI - PubMed
    1. Rajan R, Krishnan R, et al. A Polarized light microscopic study to comparatively evaluate four remineralizing agents on enamel viz CPP–ACPF, ReminPro, SHY-NM and colgate strong teeth. Int J Clin Pediatr Dent. 2015 Jan;8(1):42. doi: 10.5005/jp-journals-10005-1281. DOI: - DOI - PMC - PubMed
    1. Rirattanapong P, Vongsavan K, et al. Effect of fluoride varnishes containing tri-calcium phosphate sources on remineralization of initial primary enamel lesions. Southeast Asian J Trop Med Public Health. 2014 Mar 1;45(2):499. - PubMed
    1. Virupaxi SG, Roshan NM, et al. Comparative Evaluation of Longevity of Fluoride Release From three Different Fluoride Varnishes–An In vitro Study. J Clin Diagn Res. 2016 Aug;10(8):ZC33. doi: 10.7860/JCDR/2016/19209.8242. DOI: - DOI - PMC - PubMed
    1. Patil N, Choudhari S, et al. Comparative evaluation of remineralizing potential of three agents on artificially demineralized human enamel: an in vitro study. J Conserv Dent. 2013 Mar 1;16(2):116. doi: 10.4103/0972-0707.108185. DOI: - DOI - PMC - PubMed

LinkOut - more resources