Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 17 (11)

Biotechnological Potential of Bacteria Isolated From the Sea Cucumber Holothuria leucospilota and Stichopus vastus From Lampung, Indonesia

Affiliations

Biotechnological Potential of Bacteria Isolated From the Sea Cucumber Holothuria leucospilota and Stichopus vastus From Lampung, Indonesia

Joko T Wibowo et al. Mar Drugs.

Abstract

In order to minimize re-discovery of already known anti-infective compounds, we focused our screening approach on understudied, almost untapped marine environments including marine invertebrates and their associated bacteria. Therefore, two sea cucumber species, Holothuria leucospilota and Stichopus vastus, were collected from Lampung (Indonesia), and 127 bacterial strains were identified by partial 16S rRNA-gene sequencing analysis and compared with the NCBI database. In addition, the overall bacterial diversity from tissue samples of the sea cucumbers H. leucospilota and S. vastus was analyzed using the cultivation-independent Illumina MiSEQ analysis. Selected bacterial isolates were grown to high densities and the extracted biomass was tested against a selection of bacteria and fungi as well as the hepatitis C virus (HCV). Identification of putative bioactive bacterial-derived compounds were performed by analyzing the accurate mass of the precursor/parent ions (MS1) as well as product/daughter ions (MS2) using high resolution mass spectrometry (HRMS) analysis of all active fractions. With this attempt we were able to identify 23 putatively known and two previously unidentified precursor ions. Moreover, through 16S rRNA-gene sequencing we were able to identify putatively novel bacterial species from the phyla Actinobacteria, Proteobacteria and also Firmicutes. Our findings suggest that sea cucumbers like H. leucospilota and S. vastus are promising sources for the isolation of novel bacterial species that produce compounds with potentially high biotechnological potential.

Keywords: anti-infective marine derived compounds; de-replication; marine bacteria; mass spectrometry; sea cucumber.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Graph shows the relative abundance of OTU on a Phylum level (HL = Holothuria leucospilota, SV = Stichopus vastus).
Figure 2
Figure 2
(A) and (C) Inhibition of HCV infectivity of extracts derived from bacterial isolates of the external sea cucumber parts; (B) and (D) Inhibition of HCV infectivity of extracts derived from bacterial isolates of the internal sea cucumber parts. NC-negative control, epigallocatechin gallate (EGCG)-positive control. Viability assay results are given in the supplementary section (Figure S4).
Figure 3
Figure 3
Logic MS2 interpretation of putative structure of: (A) valinomycin (m/z 1128.66 [M + NH4]+) and (B) its derivate (m/z 1142.67 [M + NH4]+). The difference between A and B is 14 Da. It might have from the substitution of valine with either isoleucine or leucine. Figure was adapted from [48].
Figure 4
Figure 4
MS2 interpretation of putative structure of kocurin (m/z 1515.373 [M + H]+). Figure was adapted from [50].
Figure 5
Figure 5
MS2 interpretation of putative structure of surfactin (1072.686 [M + Na]+). Figure was adapted from [51].
Figure 6
Figure 6
MS2 interpretation of putative structure of plantazolicin A m/z 1336.478 [M + H]+. Figure was adapted from [52].

Similar articles

See all similar articles

References

    1. Bordbar S., Anwar F., Saari N. High-value components and bioactives from sea cucumbers for functional foods—A review. Mar. Drugs. 2011;9:1761–1805. doi: 10.3390/md9101761. - DOI - PMC - PubMed
    1. Guo Y., Ding Y., Xu F., Liu B., Kou Z., Xiao W., Zhu J. Systems pharmacology-based drug discovery for marine resources: An example using sea cucumber (Holothurians) J. Ethnopharmacol. 2015;165:61–72. doi: 10.1016/j.jep.2015.02.029. - DOI - PubMed
    1. Masre S.F., Yip G.W., Sirajudeen K.N.S., Ghazali F.C. Wound healing activity of total sulfated glycosaminoglycan (GAG) from Stichopus vastus and Stichopus hermanni integumental tissue in rats. Int. J. Mol. Med. Adv. Sci. 2010;6:49–53. doi: 10.3923/ijmmas.2010.49.53. - DOI
    1. Abedin M.Z., Karim A.A., Latiff A.A., Gan C.-Y., Ghazali F.C., Zzaman W., Hossain M.M., Ahmed F., Absar N., Sarker M.Z.I. Physicochemical and biochemical properties of pepsin-solubilized collagen isolated from the integument of sea cucumber (Stichopus vastus) J. Food Process. Preserv. 2014;38:2027–2036. doi: 10.1111/jfpp.12180. - DOI
    1. Abedin M., Karim A., Gan C., Ghazali F., Barzideh Z., Zzaman W., Zaidul I. Identification of angiotensin I converting enzyme inhibitory and radical scavenging bioactive peptides from sea cucumber (Stichopus vastus) collagen hydrolysates through optimization. Int. Food Res. J. 2015;22:1074–1082.
Feedback