Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli

Nat Commun. 2019 Nov 12;10(1):5131. doi: 10.1038/s41467-019-13152-y.

Abstract

Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene locus to the nucleoid periphery correlates with transcription, driven by either E. coli RNAP or T7 RNAP, and the effect is potentiated by translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Nucleus / genetics*
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / genetics*
  • Genetic Loci*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Models, Genetic
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Ribosomes / metabolism
  • Transcription, Genetic*
  • Viral Proteins / metabolism

Substances

  • Membrane Proteins
  • RNA, Messenger
  • Viral Proteins
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases