Morphological, cytological and genetic analyzes of the 'sango' mutant with the defects in basidiocarp development in edible mushroom Pleurotus pulmonarius

FEMS Microbiol Lett. 2019 Sep 1;366(18):fnz227. doi: 10.1093/femsle/fnz227.


A spontaneous, morphological variation 'sango' was observed in the progeny of a Pleurotus pulmonarius (Fr.) Quél. wild-type basidiocarp (also known as fruiting body) collected from the field. This variant developed wart- and coral-like structures instead of normal basidiocarps. Microscopic analysis showed that the sango phenotype had defects in the differentiation of the pileus and hymenium. Basidiocarp phenotypic data analysis in the progenies revealed that the sango trait is a heritable mutation character controlled by a single recessive gene. This mutation locus was mapped on linkage group III of a previously constructed genetic linkage map by amplified fragment length polymorphism (AFLP) technique in P. pulmonarius. Four AFLP markers identified by bulked segregant analysis showed linkage to the sango mutation locus, with the genetic distance ranging from 0 to 2.1 cM. Of these markers, one marker was co-segregated with the sango mutation locus. This knowledge will be a useful foundation for practical breeding as well as for elucidating molecular mechanisms in basidiocarp development of main edible mushrooms.

Keywords: Pleurotus pulmonarius; AFLP; basidiocarp development; bulked segregant analysis; linkage analysis; morphological mutation.

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis
  • Fruiting Bodies, Fungal / genetics*
  • Fruiting Bodies, Fungal / metabolism
  • Fruiting Bodies, Fungal / ultrastructure
  • Genes, Fungal*
  • Genes, Recessive*
  • Genetic Linkage
  • Genetic Loci
  • Genetic Markers
  • Mutation*
  • Phenotype
  • Pleurotus / genetics*
  • Pleurotus / metabolism
  • Pleurotus / ultrastructure
  • Quantitative Trait, Heritable*


  • Genetic Markers