miR-21 and miR29-a: Potential Molecular Biomarkers for HPV Genotypes and Cervical Cancer Detection

Microrna. 2020;9(4):271-275. doi: 10.2174/2211536609666191115110015.

Abstract

Background and objective: This study aimed to assess miR21 and miR-29a diagnostic usefulness for HPV infection and Cervical Cancer (CC) detection.

Methods: miRs extraction has been performed on HPV-positive, CC and negative control of 43, 50 and 46 individuals, respectively. The expression level of miRs has been analyzed using Real-Time RT-PCR.

Results: There was a correlation between the expression of miRs and the Receiver Operating Characteristic (ROC) analysis in CC and HPV-positive patients.

Conclusion: The correlation between expressions of miRs is notable. ROC curve analysis could be applied in molecular diagnostic exposure for CC related to HPV infections.

Keywords: Biomarker; HPV; Receiver Operating Characteristic (ROC); cervical cancer; miR-21; miR-29a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Alphapapillomavirus / isolation & purification*
  • Biomarkers, Tumor / genetics
  • Female
  • Humans
  • MicroRNAs / genetics*
  • Middle Aged
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / genetics
  • ROC Curve
  • Sensitivity and Specificity
  • Uterine Cervical Neoplasms / diagnosis*
  • Uterine Cervical Neoplasms / genetics
  • Young Adult

Substances

  • Biomarkers, Tumor
  • MIRN21 microRNA, human
  • MIRN29a microRNA, human
  • MicroRNAs