Metabolite profile based on 1H NMR of broiler chicken breasts affected by wooden breast myodegeneration

Ying Wang; Yang Yang; Daodong Pan; Jun He; Jinxuan Cao; Hongfei Wang; Per Ertbjerg

doi:10.1016/j.foodchem.2019.125852

Metabolite profile based on ¹H NMR of broiler chicken breasts affected by wooden breast myodegeneration

Food Chem. 2020 Apr 25:310:125852. doi: 10.1016/j.foodchem.2019.125852. Epub 2019 Nov 6.

Authors

Ying Wang¹, Yang Yang², Daodong Pan¹, Jun He², Jinxuan Cao³, Hongfei Wang⁴, Per Ertbjerg⁵

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Department of Food and Nutrition, University of Helsinki, Helsinki 00014, Finland. Electronic address: caojinxuan@nbu.edu.cn.
⁴ Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China.
⁵ Department of Food and Nutrition, University of Helsinki, Helsinki 00014, Finland. Electronic address: per.ertbjerg@helsiki.fi.

PMID: 31735464
DOI: 10.1016/j.foodchem.2019.125852

Abstract

The objective was to characterize the effect of wooden breast (WB) myodegeneration on the metabolite profile of chicken meat by ¹H NMR and multivariate data analysis. The results displayed that the metabonome of chicken breast consisted predominantly of 30 metabolites, including amino acids, organic acids, carbohydrates, alkaloids, nucleosides and their derivatives. WB-affected samples showed higher leucine, valine, alanine, glutamate, lysine, lactate, succinate, taurine, glucose, and 5'-IMP levels, but lower histidine, β-alanine, acetate, creatine, creatinine, anserine and nicotinamide adenine dinucleotide levels compared to normal fillets (p < 0.05). In conclusion, results indicated that WB-affected fillets possessed a unique biochemical signature. This unique profile could identify candidate biomarkers for diagnostic utilization and provide mechanistic insight into biochemical processes leading to WB myopathy in commercial broiler chickens.

Keywords: Chicken breast; Metabolite profile; Multivariate data analysis; NMR; Wooden breast.

MeSH terms

Amino Acids / analysis
Animals
Anserine / analysis
Carbohydrates / analysis
Chickens / metabolism
Creatine / analysis
Muscular Diseases / metabolism*
NAD / analysis
Pectoralis Muscles / chemistry*
Poultry Products / analysis*
Proton Magnetic Resonance Spectroscopy / methods*

Substances

Amino Acids
Carbohydrates
NAD
Anserine
Creatine