Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome

J Proteome Res. 2020 Jan 3;19(1):18-27. doi: 10.1021/acs.jproteome.9b00599. Epub 2019 Nov 25.

Abstract

The PAQosome is an 11-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5' UTR of ASNSD1 mRNA, encodes the 12th subunit of the PAQosome. ASDURF displays significant structural homology to β-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module is presented. The data presented here provide an example of a eukaryotic uORF-encoded polypeptide whose function is not limited to cis-acting translational regulation of downstream coding sequence and highlights the importance of including alternative ORF products in proteomic studies.

Keywords: AP-MS; ASDURF; BioID; PAQosome; R2TP/PFDL; chaperone; prefoldin; protein complex assembly; small ORF encoded peptide; uORF.

Publication types

  • Research Support, Non-U.S. Gov't

Grant support