Preparation of Antioxidant Peptide by Microwave- Assisted Hydrolysis of Collagen and Its Protective Effect Against H2O2-Induced Damage of RAW264.7 Cells

Mar Drugs. 2019 Nov 14;17(11):642. doi: 10.3390/md17110642.


Antioxidant peptides have elicited interest for the versatility of their use in the food and pharmaceutical industry. In the current study, antioxidant peptides were prepared by microwave-assisted alkaline protease hydrolysis of collagen from sea cucumber (Acaudina molpadioides). The results showed that microwave irradiation significantly improved the degree of hydrolysis of collagen and the hydroxyl radical (OH⋅) scavenging activity of hydrolysate. The content and OH⋅ scavenging activity of collagen peptides with molecular weight ≤ 1 kDa (CPS) in the hydrolysate obtained at 250 W increased significantly compared with the non-microwave-assisted control. CPS could scavenge OH⋅ and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in a dose-dependent manner. The scavenging activity of OH⋅ and DPPH radical was 93.1% and 41.2%, respectively, at CPS concentration of 1 mg/mL. CPS could significantly promote RAW264.7 cell proliferation and reduce the Reactive Oxygen Species (ROS) level of H2O2-induced damage in RAW264.7 cells in a dose-dependent manner. Furthermore, all CPS-treated groups exhibited an increase in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and a decrease in malondialdehyde (MDA) level compared with the control. These results showed that CPS could effectively protect RAW264.7 cells from H2O2-induced damage, implying the potential utilization of CPS as a natural antioxidant for food and pharmaceutical applications.

Keywords: Acaudina molpadioides; antioxidant peptides; microwave-assisted hydrolysis; oxidative stress protection.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Biphenyl Compounds / metabolism
  • Cell Line
  • Cell Proliferation / drug effects
  • Collagen / metabolism*
  • Glutathione Peroxidase / metabolism
  • Hydrogen Peroxide / pharmacology*
  • Hydrolysis
  • Hydroxyl Radical / metabolism
  • Malondialdehyde / metabolism
  • Mice
  • Microwaves
  • Oxidative Stress / drug effects
  • Peptides / pharmacology*
  • Protective Agents / pharmacology*
  • RAW 264.7 Cells
  • Reactive Oxygen Species / metabolism
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Biphenyl Compounds
  • Peptides
  • Protective Agents
  • Reactive Oxygen Species
  • diphenyl
  • Hydroxyl Radical
  • Malondialdehyde
  • Collagen
  • Hydrogen Peroxide
  • Glutathione Peroxidase
  • Superoxide Dismutase