Cell Biology: Hacking Alpha Satellites out of the HAC

Alexander B Willis; Daniel R Foltz

doi:10.1016/j.cub.2019.09.059

Cell Biology: Hacking Alpha Satellites out of the HAC

Curr Biol. 2019 Nov 18;29(22):R1194-R1196. doi: 10.1016/j.cub.2019.09.059.

Authors

Alexander B Willis¹, Daniel R Foltz²

Affiliations

¹ Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
² Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address: dfoltz@northwestern.edu.

PMID: 31743678
DOI: 10.1016/j.cub.2019.09.059

Abstract

Human artificial chromosomes (HACs) are a potentially powerful technique for genomic engineering, but their use is limited by the repetitive centromeric alpha-satellite DNA needed to form a centromere. A new study presents a method to induce HAC centromere formation on non-repetitive templates through sequence-directed CENP-A nucleosome seeding.

Publication types

Comment

MeSH terms

Centromere
Centromere Protein A
Centromere Protein B
Chromosomes, Artificial, Human*
DNA, Satellite
Humans

Substances

Centromere Protein A
Centromere Protein B
DNA, Satellite