Abnormal Photic Entrainment to Phase-Delaying Stimuli in the R6/2 Mouse Model of Huntington's Disease, despite Retinal Responsiveness to Light

eNeuro. 2019 Dec 10;6(6):ENEURO.0088-19.2019. doi: 10.1523/ENEURO.0088-19.2019. Print 2019 Nov/Dec.

Abstract

The circadian clock located in the suprachiasmatic nucleus (SCN) in mammals entrains to ambient light via the retinal photoreceptors. This allows behavioral rhythms to change in synchrony with seasonal and daily changes in light period. Circadian rhythmicity is progressively disrupted in Huntington's disease (HD) and in HD mouse models such as the transgenic R6/2 line. Although retinal afferent inputs to the SCN are disrupted in R6/2 mice at late stages, they can respond to changes in light/dark cycles, as seen in jet lag and 23 h/d paradigms. To investigate photic entrainment and SCN function in R6/2 mice at different stages of disease, we first assessed the effect on locomotor activity of exposure to a 15 min light pulse given at different times of the day. We then placed the mice under five non-standard light conditions. These were light cycle regimes (T-cycles) of T21 (10.5 h light/dark), T22 (11 h light/dark), T26 (13 h light/dark), constant light, or constant dark. We found a progressive impairment in photic synchronization in R6/2 mice when the stimuli required the SCN to lengthen rhythms (phase-delaying light pulse, T26, or constant light), but normal synchronization to stimuli that required the SCN to shorten rhythms (phase-advancing light pulse and T22). Despite the behavioral abnormalities, we found that Per1 and c-fos gene expression remained photo-inducible in SCN of R6/2 mice. Both the endogenous drift of the R6/2 mouse SCN to shorter periods and its inability to adapt to phase-delaying changes will contribute to the HD circadian dysfunction.

Keywords: Huntington's disease; jetlag; light therapy; phase response curve; phase shift; photic entrainment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circadian Rhythm / physiology*
  • Disease Models, Animal
  • Gene Expression Regulation
  • Huntington Disease / metabolism
  • Huntington Disease / physiopathology*
  • Mice
  • Motor Activity / physiology*
  • Neurons / metabolism
  • Period Circadian Proteins / genetics
  • Period Circadian Proteins / metabolism
  • Photic Stimulation
  • Photoperiod*
  • Proto-Oncogene Proteins c-fos / genetics
  • Proto-Oncogene Proteins c-fos / metabolism
  • Retina / metabolism
  • Retina / physiopathology*
  • Suprachiasmatic Nucleus / metabolism
  • Suprachiasmatic Nucleus / physiopathology*

Substances

  • Per1 protein, mouse
  • Period Circadian Proteins
  • Proto-Oncogene Proteins c-fos