Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 24 (1), 67-75

Therapeutic Use of Adipose-Derived Stromal Cells in a Murine Model of Acute Pancreatitis

Affiliations

Therapeutic Use of Adipose-Derived Stromal Cells in a Murine Model of Acute Pancreatitis

Alexandra M Roch et al. J Gastrointest Surg.

Abstract

Background: No specific therapy exists for acute pancreatitis (AP), and current treatment remains entirely supportive. Adipose stem cells (ASCs) have significant immunomodulatory and regenerative activities. We hypothesized that systemic administration of ASCs would mitigate inflammation in AP.

Methods: AP was induced in mice by 6 hourly intraperitoneal injections of cerulein. Twenty-four hours after AP induction, mice were randomized into four systemic treatment groups: sham group (no acute pancreatitis), vehicle, human ASCs, and human ASC-conditioned media. Mice were sacrificed at 48 h, and blood and organs were collected and analyzed. Pancreatic injury was quantified histologically using a published score (edema, inflammation, and necrosis). Pancreatic inflammation was also studied by immunohistochemistry and PCR.

Results: When using IV infusion of Hoechst-labeled ASCs, ASCs were found to localize to inflamed tissues: lungs and pancreas. Mice treated with ASCs had less severe AP, as shown by a significantly decreased histopathology score (edema, inflammation, and necrosis) (p = 0.001). ASCs infusion polarized pancreatic macrophages toward an anti-inflammatory M2 phenotype. ASC-conditioned media reduced pancreatic inflammation similarly to ASCs only, highlighting the importance of ASCs secreted factors in modulating inflammation.

Conclusion: Intravenous delivery of human ASCs markedly reduces pancreatic inflammation in a murine model of AP ASCs which represent an effective therapy for AP.

Keywords: Acute pancreatitis; Adipose stem cells; Murine model; Treatment.

Similar articles

See all similar articles

References

    1. Int J Clin Exp Pathol. 2014 Jun 15;7(7):3580-95 - PubMed

References

    1. Tissue Eng. 2001 Apr;7(2):211-28 - PubMed

References

    1. Stem Cells Int. 2019 May 2;2019:5858247 - PubMed

References

    1. Nephrol Dial Transplant. 1993;8(10):1079-84 - PubMed

References

    1. N Engl J Med. 2010 Apr 22;362(16):1491-502 - PubMed

References

    1. Gastroenterology. 2001 Jul;121(1):184-94 - PubMed

References

    1. Pancreas. 2003 Oct;27(3):253-60 - PubMed

References

    1. Stem Cells Int. 2015;2015:306836 - PubMed

References

    1. Pancreas. 2016 Oct;45(9):1282-1293 - PubMed

References

    1. Lancet. 2008 Jan 12;371(9607):143-52 - PubMed

References

    1. Gut. 2013 Jan;62(1):102-11 - PubMed

References

    1. Heart Lung Circ. 2008 Oct;17(5):395-403 - PubMed

References

    1. Circ Res. 2009 Jun 19;104(12):1410-20 - PubMed

References

    1. JOP. 2007 Mar 10;8(2):177-85 - PubMed

References

    1. Stem Cells Int. 2016;2016:7174319 - PubMed

References

    1. Am J Gastroenterol. 2006 Oct;101(10):2379-400 - PubMed

References

    1. Gastroenterology. 1985 May;88(5 Pt 1):1192-204 - PubMed

References

    1. Arch Pharm Res. 2015;38(5):742-51 - PubMed

References

    1. Keio J Med. 2005 Sep;54(3):132-41 - PubMed

References

    1. Ann Rheum Dis. 2017 Jan;76(1):196-202 - PubMed

References

    1. HPB (Oxford). 2006;8(4):264-86 - PubMed

References

    1. Gastroenterology. 2000 Mar;118(3):582-90 - PubMed

References

    1. Chest. 2003 Dec;124(6):2341-51 - PubMed

References

    1. J Vet Sci. 2016 Dec 30;17(4):539-548 - PubMed

References

    1. Gastroenterology. 2011 Mar;140(3):998-1008 - PubMed

References

    1. Gastroenterology. 2003 Mar;124(3):725-36 - PubMed

References

    1. Pancreas. 2016 May-Jun;45(5):707-13 - PubMed

References

    1. World J Gastroenterol. 2010 Aug 28;16(32):3995-4002 - PubMed

References

    1. Ann Epidemiol. 2007 Jul;17(7):491-7 - PubMed

References

    1. J Invasive Cardiol. 2006 Nov;18(11):552-6 - PubMed

References

    1. Lancet. 2004 May 1;363(9419):1439-41 - PubMed

References

    1. N Engl J Med. 1994 Apr 28;330(17):1198-210 - PubMed

References

    1. Cytotherapy. 2014 Feb;16(2):245-57 - PubMed

References

    1. Brain Res. 2005 Feb 21;1035(1):73-85 - PubMed

References

    1. J Gastrointest Surg. 2019 Sep;23(9):1834-1839 - PubMed

References

    1. Stem Cells Int. 2018 Jun 14;2018:3057624 - PubMed

References

    1. Cytotherapy. 2006;8(4):315-7 - PubMed

References

    1. Circulation. 2004 Mar 16;109(10):1292-8 - PubMed

References

    1. Stem Cells Transl Med. 2013 Jun;2(6):455-63 - PubMed

References

    1. Cytotherapy. 2013 Feb;15(2):154-62 - PubMed

References

    1. Science. 1999 Apr 2;284(5411):143-7 - PubMed

References

    1. Stem Cell Res Ther. 2017 Jan 23;8(1):12 - PubMed

References

    1. Stem Cell Reports. 2014 Feb 06;2(2):171-9 - PubMed

References

    1. Am J Physiol. 1984 Apr;246(4 Pt 1):G457-67 - PubMed

References

    1. Intensive Care Med. 1977 Aug;3(2):97-8 - PubMed

References

    1. Cell Transplant. 2010;19(6):667-79 - PubMed

References

    1. J Transl Med. 2015 Feb 21;13:67 - PubMed

References

    1. Pancreas. 2007 Nov;35(4):302-7 - PubMed

References

    1. Am J Physiol Gastrointest Liver Physiol. 2005 Jun;288(6):G1259-65 - PubMed

References

    1. Am J Physiol Gastrointest Liver Physiol. 2008 Sep;295(3):G552-8 - PubMed

References

    1. World J Gastroenterol. 2012 May 14;18(18):2270-9 - PubMed

References

    1. Surgery. 1996 Sep;120(3):515-21 - PubMed

References

    1. Kidney Int. 2005 Oct;68(4):1613-7 - PubMed

References

    1. Hepatology. 1998 Nov;28(5):1282-8 - PubMed

References

    1. Gut. 2000 Oct;47(4):546-52 - PubMed

References

    1. Crit Care Med. 1995 May;23(5):901-8 - PubMed

References

    1. Sci Rep. 2016 Dec 05;6:38438 - PubMed

References

    1. Stem Cells Transl Med. 2015 Jun;4(6):598-602 - PubMed

References

    1. Gastroenterology. 1997 Nov;113(5):1741-6 - PubMed

References

    1. Am J Transl Res. 2011 Feb;3(2):159-65 - PubMed

References

    1. J Clin Invest. 1997 Oct 15;100(8):2022-7 - PubMed

References

    1. Int J Clin Exp Pathol. 2013 Nov 15;6(12):2703-12 - PubMed

References

    1. Virchows Arch A Pathol Anat Histol. 1977 Mar 11;373(2):97-117 - PubMed

LinkOut - more resources

Feedback