Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila

Biogerontology. 2020 Apr;21(2):155-171. doi: 10.1007/s10522-019-09849-8. Epub 2019 Nov 20.

Abstract

Mitochondrial alternative NADH dehydrogenase (aNDH) was found to extend lifespan when expressed in the fruit fly. We have found that fruit flies expressing aNDH from Ciona intestinalis (NDX) had 17-71% lifespan prolongation on media with different protein-tocarbohydrate ratios except NDX-expressing males that had 19% shorter lifespan than controls on a high protein diet. NDX-expressing flies were more resistant to organic xenobiotics, 2,4-dichlorophenoxyacetic acid and alloxan, and inorganic toxicant potassium iodate, and partially to sodium molybdate treatments. On the other hand, NDX-expressing flies were more sensitive to catechol and sodium chromate. Enzymatic analysis showed that NDX-expressing males had higher glucose 6-phosphate dehydrogenase activity, whilst both sexes showed increased glutathione S-transferase activity.

Keywords: Antioxidant defence; Drosophila melanogaster; Mitochondria; Reactive oxygen species; Stress resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Ciona intestinalis / enzymology*
  • Ciona intestinalis / genetics
  • Drosophila melanogaster / drug effects*
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics
  • Drug Resistance* / genetics
  • Energy Metabolism* / genetics
  • Female
  • Gene Expression Regulation
  • Longevity* / genetics
  • Male
  • NADH Dehydrogenase / genetics
  • NADH Dehydrogenase / metabolism*
  • Sex Factors
  • Xenobiotics / pharmacology*

Substances

  • Xenobiotics
  • NADH Dehydrogenase