Oral acute and sub-acute toxic effects of hydroalcoholic Terminalia chebula Retz and Achillea wilhelmsii extracts in BALB/c mice

Biomedicine (Taipei). 2019 Dec;9(4):25. doi: 10.1051/bmdcn/2019090425. Epub 2019 Nov 21.


Background: This study examined the acute and sub-acute toxic effects of Terminalia chebula and Achillea wilhelmsii extracts on the murine model.

Methods: In both phases, mice were assigned to intervention and control groups. At the end of study, the liver, kidney, and heart tissues were collected for histopathological studies.

Results: In the acute phase of the study, the safe dose was ≤5000 mg/kg for both extracts. In sub-acute phase, LD50 (95% CI) of Achillea wilhelmsii extract was determined ≥5000 mg/kg and that of Terminalia chebula extract 2754.436 (2438-3114) mg/kg. The highest dose of T. chebula extract induced few histopathological changes.

Conclusion: It will be useful to gain information on the minimum lethal doses of T. chebula and A. wilhelmsii to adopt safe doses of the two plants.

Keywords: Achillea wilhelmsii; Acute toxicity; Animal Model; Sub-acute toxicity; Terminalia chebula.