Silencing of long non-coding RNA H19 downregulates CTCF to protect against atherosclerosis by upregulating PKD1 expression in ApoE knockout mice

Aging (Albany NY). 2019 Nov 22;11(22):10016-10030. doi: 10.18632/aging.102388. Epub 2019 Nov 22.

Abstract

This study aimed to explore the interactions among long non-coding RNA H19, transcriptional factor CCCTC-binding factor (CTCF) and polycystic kidney disease 1 (PKD1), and to investigate its potentially regulatory effect on vulnerable plaque formation and angiogenesis of atherosclerosis. We established an atherosclerosis mouse model in ApoE knockout mice, followed by gain- and loss-of-function approaches. H19 was upregulated in aortic tissues of atherosclerosis mice, but silencing of H19 significantly inhibited atherosclerotic vulnerable plaque formation and intraplaque angiogenesis, accompanied by a downregulated expression of MMP-2, VEGF, and p53 and an upregulated expression of TIMP-1. Moreover, opposite results were found in the aortic tissues of atherosclerosis mice treated with H19 or CTCF overexpression. H19 was capable of recruiting CTCF to suppress PKD1, thus promoting atherosclerotic vulnerable plaque formation and intraplaque angiogenesis in atherosclerosis mice. The present study provides evidence that H19 recruits CTCF to downregulate the expression of PKD1, thereby promoting vulnerable plaque formation and intraplaque angiogenesis in mice with atherosclerosis.

Keywords: CCCTC-binding factor; atherosclerosis; atherosclerotic vulnerable plaque formation; long non-coding RNA H19; polycystic kidney disease 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / metabolism*
  • Atherosclerosis / genetics
  • Atherosclerosis / metabolism*
  • CCCTC-Binding Factor / genetics
  • CCCTC-Binding Factor / metabolism*
  • Disease Models, Animal
  • Down-Regulation*
  • Gene Silencing
  • Mice
  • Mice, Knockout, ApoE
  • Plaque, Atherosclerotic / genetics
  • Plaque, Atherosclerotic / metabolism
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Up-Regulation*

Substances

  • CCCTC-Binding Factor
  • H19 long non-coding RNA
  • RNA, Long Noncoding
  • protein kinase D
  • Protein Kinase C