Binary magnetic metal-organic frameworks composites: a promising affinity probe for highly selective and rapid enrichment of mono- and multi-phosphopeptides

Mikrochim Acta. 2019 Nov 22;186(12):832. doi: 10.1007/s00604-019-3916-5.

Abstract

A binary magnetic metal-organic framework (MOF)-functionalized material (magG@PDA@Ni-MOF@Fe-MOF) was prepared through grafting Ni-MOF and Fe-MOF on magnetic (Fe3O4) graphene with polydopamine (PDA) as a middle layer. Compared with single MOFs functionalized materials (magG@PDA@Ni-MOF and magG@PDA@Fe-MOF) under the same conditions, magG@PDA@Ni-MOF@Fe-MOF not only displays lower detection limits (4 fmol) and selectivity (1:1000), but also has better enrichment efficiency for both multi- and monophosphopeptides. Other than this, magG@PDA@Ni-MOF@Fe-MOF exhibits fine reusability (five cycles) and rapid enrichment property (1 min), and 24 phosphopeptides were detected when it was applied to the analysis of human saliva. Graphical abstractThe strategy of preparing a binary magnetic metal-organic framework (MOF)-functionalized material (magG@PDA@Ni-MOF@Fe-MOF) through grafting Ni-MOF and Fe-MOF on magnetic graphene with polydopamine (PDA) as a middle layer.

Keywords: Immobilized metal ion affinity chromatography (IMAC); MALDI-TOF mass spectrometry; MagG@PDA@Ni-MOF@Fe-MOF; Metal-organic frameworks (MOFs); Phosphopeptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemistry*
  • Humans
  • Magnetic Phenomena
  • Metal-Organic Frameworks / chemistry*
  • Particle Size
  • Phosphopeptides / analysis*
  • Saliva / chemistry
  • Surface Properties

Substances

  • Fluorescent Dyes
  • Metal-Organic Frameworks
  • Phosphopeptides