Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity

E Yu Parshina; A I Yusipovich; A R Brazhe; M A Silicheva; G V Maksimov

doi:10.1007/s10867-019-09533-5

Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity

J Biol Phys. 2019 Dec;45(4):367-377. doi: 10.1007/s10867-019-09533-5. Epub 2019 Nov 22.

Authors

E Yu Parshina¹, A I Yusipovich², A R Brazhe², M A Silicheva², G V Maksimov²

Affiliations

¹ Biological Department, M.V. Lomonosov Moscow State University, Leninskie gory, 1-12, 119234, Moscow, Russia. parshinae@gmail.com.
² Biological Department, M.V. Lomonosov Moscow State University, Leninskie gory, 1-12, 119234, Moscow, Russia.

Abstract

The intensity of erythrocyte membrane fluctuations was studied by laser interference microscopy (LIM), which provide information about mechanical properties of the erythrocyte membrane. Atomic force microscopy (AFM) was used to study erythrocyte surface relief; it is related to the cytoskeleton structure of erythrocyte membrane. Intact human erythrocytes and erythrocytes with a destroyed cytoskeleton were used. According to the obtained results, cytoskeleton damage induced by heating up to 50 °С results in a reduced intensity of cell membrane fluctuations compared to non-treated cells (20.6 ± 10.2 vs. 30.5 ± 5.5 nm, correspondingly), while the roughness of the membrane increases (4.5 ± 1.5 vs. 3.4 ± 0.5 nm, correspondingly).

Keywords: Cytoskeleton; Elastic properties; Erythrocytes; Membrane fluctuations; Roughness.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomechanical Phenomena
Cytoskeleton / metabolism*
Erythrocyte Membrane / metabolism*
Erythrocytes / cytology*
Hot Temperature / adverse effects*
Mechanical Phenomena*